SKIN PROTECTION 85


14 12 10 8 6 4 2 0


Control Control + UD +30%


600 500 400 300 200 100 0


Control + UD + BIOLIME


Figure 7: Adenosine triphosphate (ATP) measurement on epidermal keratinocytes. ANOVA, significant test p<0.05 (n=3).


vulnerable to pollution, since they are damaged in several ways, suggesting that this central organelle may be targeted early in this toxic process. Mitochondria are organelles involved in several cutaneous functions and structure. Dysregulated mitochondria could contribute to skin disorders and ageing.5


Indeed, pollution


may affect the mitochondrial membrane potential and decrease cardiolipin content.6 Furthermore, pollution inhibits oxidative phosphorylation, the activity of complexes I and III and decreases the number of oligomycin-binding sites, which may indicate interactions with ATP synthase.7 Under pollution and stress conditions,


ATP production decreases, thus decreasing cell efficiency. Pollution also degrades DNA, deteriorating genetic code and


Control


+340%


Control + UD


Control + UD + BIOLIME


Figure 6: Olive Tail Moment measurement on epidermal keratinocytes. ANOVA, significant test p<0.01 (n=3).


protein synthesis. The lime extract fights against pollution by increasing ATP production and decreasing DNA damage. Protection of mitochondria against urban pollution seems to be a key factor of skin preservation, especially extra mitochondria oxidative stress and maintenance of mitochondria ATP synthesis.


For the first time, Biolie has set up an original test to evaluate the effect of oxidative stress (extra-mitochondrial stress) induced by Urban Dust, representative of urban pollution, on keratinocytes. Olive Tail Moment on cell DNA has been used as parameter to monitor the exposition to Urban Dust. In the same way, Biolie performed a study to evaluate the protection of the lime extract on mitochondria ATP synthesis submitted to urban pollution.


Mitochondria protection The comet test (or ‘single cell gel electrophoresis assay’) can measure the amount of damaged DNA in a contaminated organism. It makes it possible to quantify damages in the DNA after exposure to mutagenic agents. The comet test is an agarose microgel electrophoresis technique for detecting individualised cell DNA fragmentation. In this study, the comet assay was performed on NHEK cells (normal human epidermal keratinocytes).


NHEK have been incubated with


0.1 mg / mL Urban Dust, with or without 1 % (v / v) the lime extract for 3 h at 37 °C under humid atmosphere at 5 % CO2


.


Urban Dust increases Olive Tail Moment on cell DNA by 340%, a sign that pollution


Viis t ussit u st gllobal


stand J130 at in-cosm


t us at smetics


April 2019


PERSONAL CARE EUROPE


ATP (µg/mL)


Olive Tail Moment


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