72 NATURALS
Furthermore, bergamot flavonoids exert activities on adipose tissue, inducing the breakdown of triglycerides (lipolysis) and reducing the adipogenesis and fat accumulation.12
Lo Furno et al, evaluated
the activity of bergamot flavonoids on mesenchymal stem cells from adipose tissue during adipogenic differentiation. It was verified the lipolytic activity either in differentiated mesenchymal stem cells and during the adipogenic differentiation stage.13
These scientific evidences encouraged Bionap to develop Flavoslim, an eco- friendly ingredient able to counteract the cutaneous orange-peel appearance and sagging skin, and to slim the silhouette. Flavoslim (INCI Name: Citrus Aurantium
Bergamia (Bergamot) Fruit Extract, Maltodextrin) is a preservative-free powdered extract, standardised in flavonoids (naringin, neoeriocitrin and neohesperidin - 8-10% HPLC) that belong to the category of flavanones glycosides. The capability of Flavoslim to reduce inflammatory mediators related to the onset of oedema, to induce lipolysis and reduce fat storages have been already tested with in vitro models. Therefore, the aim of the work
presented hereafter was to clinically evaluate the action of Flavoslim (now referred to as ‘Citrus bergamia extract’) in counteracting the signs related to the cellulite and improving body silhouette. A randomised, double-blind and placebo-controlled clinical evaluation was carried out on 20 female volunteers, aged 18 to 55, presenting mild to moderate cellulite-derived skin imperfections. Enrolled panellists applied twice-daily a formula with Citrus bergamia extract at 1.5%, and a placebo formula (half-body application).
The study lasted 8 weeks and product
effects on skin were assessed after 14, 28 and 56 days. Instrumental techniques were employed to assess the activity of the extract on microcirculation, on skin elasticity
Mean reduction of SAT thickness on thighs 0
-0.1 -0.2 -0.3 -0.4 -0.5 -0.6
T14
Time (dd) T28
T56
Results The thermographic analysis highlighted that the formulation with Citrus bergamia extract improved the thermal homogeneity
Mean reduction of SAT thickness on gluteus 0 -0.16 -0.17 -0.16 -0.19 -0.45** -0.56** Placebo Citrus bergamia ext.
Figure 4: The graph reports the mean variation versus T0 at each checkpoint for the SAT thickness on thighs.
PERSONAL CARE EUROPE
-0.1 -0.2 -0.3 -0.4 -0.5 -0.6 -0.7
T14
Time (dd) T28
T56
Figure 3: Picture shows the improvement of thermal homogeneity which is linked to the decrease of the different coloured areas. The more the thermal homogeneity is, the better the microcirculation is.
and on adipose tissue. Furthermore, slimming effects were evaluated through the measurements of thighs and arms contour. The instrumental assessments were integrated by expert (dermatologist) scoring and self-assessment of the subjects participating to the study. Collected data were submitted to two-way Student’s t-test for paired data. We demonstrated that Citrus bergamia extract is an effective ingredient to counteract body imperfections, enhance skin firmness and slim the silhouette.
Effect of Citrus bergamiaextract on blood and lymphatic microcirculation Design
Cellulite-derived blood and lymphatic microcirculation alterations were objectively measured by means of liquid crystal thermography and image analysis. Skin thermal images were acquired on thighs using an infrared thermal camera. The output of the technique is the distribution of cold and warm areas inside the cutaneous zones affected by the cellulite. Collected data are reported as percentage of subjects that showed an improvement of thermal homogeneity. Acquired images showed the distribution of temperature over time and here we report one of the best cases.
after 14 days in 30% of panellists, whereas the placebo formula produced an improvement in just 15% of panellists. A 28-day treatment caused an
improvement in thermal homogeneity in 55% of volunteers who applied the active formula, versus a 35% who applied the placebo formula. After 56 days, active formula produced a better thermal homogeneity in 80% of panellists, whereas placebo formula caused the same result in 45% of subjects. These results underline the remarkable activity of bergamot flavonoids on blood and lymphatic microcirculation. Figure 3 shows that, starting from t0 to t56, the number of different coloured areas, which represent different cutaneous temperatures, a symptom of microcirculation deficit, decreases.
Lipolytic activity of Citrus bergamia extract: Superficial adipose tissue (SAT) thickness measurement Design
The superficial adipose tissue (SAT) thickness was measured using an ultrasound-based imaging technique. The technique allows users to visualise the adipose tissue distribution and to measure fat thickness inside the skin area affected by the cellulite.
Ultrasound imaging works by using -0.19 -0.19 -0.21 -0.23 -0.47** -0.59*** Placebo Citrus bergamia ext.
Figure 5: The graph reports the mean variation versus T0 at each checkpoint for the SAT thickness on gluteus.
high frequency sound waves and their echoes in order to obtain images inside the human body. A transducer probe is used to generate the sound pulses and transmit them into the body. The sound waves travel into the body and are strongly reflected at interfaces between different types of tissue such as fat and muscle, or muscle and bone. At each interface a fraction of the sound wave is reflected and the rest transmitted through the interface to penetrate further into the tissue. Recording the reflected sound wave echoes, an image is produced and it is converted to tissue thickness by measuring the time the signal takes to reach the interface and multiplying the time by the sound speed through tissue. The measurements were carried out on thighs, gluteus, hips, and arms at 14, 28 and 56 days. Data are expressed in mm and
intergroup (active vs. placebo) statistical April 2019
Variation vs T0
Variation vs T0
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92 |
Page 93 |
Page 94 |
Page 95 |
Page 96 |
Page 97 |
Page 98 |
Page 99 |
Page 100 |
Page 101 |
Page 102 |
Page 103 |
Page 104 |
Page 105 |
Page 106 |
Page 107 |
Page 108 |
Page 109 |
Page 110 |
Page 111 |
Page 112 |
Page 113 |
Page 114 |
Page 115 |
Page 116 |
Page 117 |
Page 118 |
Page 119 |
Page 120 |
Page 121 |
Page 122 |
Page 123 |
Page 124 |
Page 125 |
Page 126 |
Page 127 |
Page 128 |
Page 129 |
Page 130 |
Page 131 |
Page 132 |
Page 133 |
Page 134 |
Page 135 |
Page 136 |
Page 137 |
Page 138 |
Page 139 |
Page 140 |
Page 141 |
Page 142 |
Page 143 |
Page 144 |
Page 145 |
Page 146 |
Page 147 |
Page 148 |
Page 149 |
Page 150 |
Page 151 |
Page 152 |
Page 153 |
Page 154 |
Page 155 |
Page 156 |
Page 157 |
Page 158 |
Page 159 |
Page 160 |
Page 161 |
Page 162 |
Page 163 |
Page 164 |
Page 165 |
Page 166 |
Page 167 |
Page 168 |
Page 169 |
Page 170 |
Page 171 |
Page 172 |
Page 173 |
Page 174 |
Page 175 |
Page 176 |
Page 177 |
Page 178 |
Page 179 |
Page 180 |
Page 181 |
Page 182 |
Page 183 |
Page 184 |
Page 185 |
Page 186 |
Page 187 |
Page 188
