SKIN CARE 37 Before After 28 days
14 12 10 8 6 4 2 0
Epidermis SLEB Dermis
Figure 6: Representative ultrasound image of the increase in epidermis + dermis density after 28 days treatment with 0.4% goji stem cell active. SLEB = subepidermal low-echogenic band.
replaced with assay medium containing or not (control) goji stem cell extract. For another series, medium was removed and replaced with the conditioned medium from MSCs (control or goji stem cell extract treated MSCs). Cells were then incubated for 24 hours. All experimental conditions were performed in n=3. At the end of incubation, cells were washed in phosphate buffered saline (PBS) solution and immediately frozen at -80°C. Total RNA was extracted from the cells and the expression of extracellular matrix genes was then assessed with RT- qPCR using the LightCycler System (Roche).
Clinical study on photoaged skin In a randomised, placebo-controlled clinical study, twenty-three Caucasian women aged between 41 and 69 years (mean age: 56 years) who displayed signs of photoageing applied either a cream with 0.4% goji stem cell active on one half of the face and one forearm and the corresponding placebo on the other half of the face and the other
Before
*p<0.05 versus initial conditions *
Placebo 0.4% Goji stem cell active
Figure 7: Decrease in wrinkle depth on crow’s feet after 56 days treatment with 0.4% goji stem cell active.
forearm, twice daily for a period of 56 days. The wrinkle depth was measured using PRIMOS lite (Canfield, USA). The density of the skin (epidermis + dermis) was determined by ultrasonic measurements in triplicates using DermaScan C (Cortex, Denmark) which counts the number of low- echogenic pixels corresponding to low- density areas on a 2-D portion of epidermis and dermis. The lower the number of dark pixels the higher the skin density.
Clinical study on oval face shape In a randomised, placebo-controlled clinical study, sixty-seven Caucasian women aged between 39 and 70 years (average age 57 years) with sagging facial skin were split into two groups. One group applied a cream with 0.4 % goji stem cell active and the other group applied the corresponding placebo cream on the entire face and neck twice daily for 28 days. The facial sagging and oval face shape was determined by measuring the size of the neck/submandibular triangle area by
After 56 days
image analysis of the pictures of the volunteers’ faces that were taken with Visioface 1000 D (Courage + Khazaka). For the image analysis, a straight line was drawn vertically through the middle of the chin. On either side of the face, vertical parallel lines were drawn to intersect at the start of the jawline/sagging skin. The points of the jawline intersection were then connected with the middle of the chin. The area below the drawn V lines was determined. A reduction of this area would suggest a reduction in facial sagging and an improved V-shape of the face.
Results and discussion
Maintenance of stemness in aged MSCs Although much slower than differentiated cells, MSCs also undergo ageing processes. This is characterised by the reduced production of stem cell markers and eventually a halt in proliferation. To investigate whether the goji stem cell active influences the vitality of MSCs, aged MSCs were treated with 1% goji stem cell extract for 72 hours. Results showed that treatment with goji stem cell extract increased the expression of the stem cell markers CD34, Oct4 and Nanog in aged MSCs (Fig 2). This means the goji stem cell active is able to help mesenchymal stem cells to maintain their stemness.
Before After 56 days
Figure 8: Representative before/after pictures of two volunteers to illustrate the decrease in wrinkle depth on crow’s feet after 56 days treatment with 0.4% goji stem cell active.
April 2019
Increase in MSC exosome production In order to assess the effect of the goji stem cell active on the exosome production capability, human MSCs were either treated or not (control) with 0.1% goji stem cell extract for a period of 24 hours. The exosomes that were released from the cells were quantified in two ways, either measuring the total protein or enzymatic activity of a known exosomal protein. Both quantification methods revealed that treatment with goji stem cell extract leads to an increase in exosome production by MSCs (Fig 3).
PERSONAL CARE EUROPE
Decrease in wrinkle depth compared to initial conditions in %
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