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TESTING 45


Higher pH values at certain body sites like the axilla or the interdigital areas promote the colonisation of certain odour- producing or harmful bacteria and fungi.28, 29 In particular, atopic dermatitis is often linked to increased pH-values and at the same time a different composition of the bacterial associations with an increased presence of Staphylococcus aureus.27


Skin


pH measurement can show whether a shift of the pH value could be obtained by the product application. There are also other measurement parameters that can be used to show a positive effect on skin health.


Conclusion Research on the skin microbiome is a fascinating and trendy topic and will probably evolve further. However, despite the intense research, the microbiome shows huge inter- and intra-individual variation and there has been until now no scientific consensus on its perfect composition. An aspect that still needs more research


effort is the influence of cosmetic products on the resident microbiome. What is happening in the microbiome realm when applying oily formulations on skin areas that by nature do not have high sebum production and feature an adapted healthy microbiome? How can we ensure that added microorganisms will survive in the microbial communities on the skin surface? And might it be possible that the well- meant application of probiotic products might have an adverse effect on the skin microbiome, destroying the adapted balance by adding more species of certain bacteria?


The findings that the skin microbiome is essential for skin health pose a challenge to cosmetic industry to develop new products that not only consider traditional skin types such as oily or dry but to widen their understanding of skin types to certain microbiome types.


Since the claim behind using


formulations modifying, enhancing or feeding the microbiome, is the improvement of skin health and especially the strengthening of the skin barrier function, traditional biophysical testing methods and imaging are well-suited to substantiate health-related claims of such products.


PC


References 1 Luckey T. Introduction to intestinal


microecology. The American Journal of Clinical Nutrition 1972; 25(12): 1292–1294


2 Bianconi E, et al. An estimation of the number of cells in the human body. Annals of Human Biology 2013; 40:5: 63–471


3 Sender R, Fuchs S, Milo R. Revised estimates for the number of human and bacteria cells in the body. 6 January, 2016, bioRxiv doi:


April 2019


Figure 9: pH-changes may influence microbial association. Their measurement can give valuable information on the efficacy of products.


http://dx.doi.org/10.1101/036103


4 NIH: NIH Human Microbiome Project defines normal bacterial makeup of the body, News Releases, 13 June 2012, https://www.nih.gov/news-events/news- releases/nih-human-microbiome-project- defines-normal-bacterial-makeup-body


5 Quing Gu, Ping Li. Biosynthesis of Vitamins by Probiotic Bacteria, Probiotics and Prebiotics in Human Nutrition and Health, Ed. Venketeshwer, 135-148, 2016


6 Olszak T, An D, Zeissig S, Vera MP, et al. Microbial exposure during early life has persistent effects on natural killer T cell function. Science 2012; 336:489–493.


7 NIH Human Microbiome Project, https://www.hmpdacc.org/overview/


8 Wallen-Russel C. Meta Analysis of Skin Microbiome: New Link between Skin Microbiota Diversity and Skin Health with Proposal to Use This as a Future Mechanism to Determine Whether Cosmetic Products Damage the Skin, Cosmetics 2017; 4(2): 14.


9 Grice E. The Skin Microbiome, Nat Rev Microbiology 2011; 9(4): 244-253.


10 Costello E, et al. Bacterial Community Variation in Human Body Habitats Across Space and Time, Science, 2009; 326 (5960): 1694-1697.


11 Grice E A, et al. Topographical and Temporal Diversity of the Human Skin Microbiome, Science 2009; 324 (5931): 1190-1192.


12 Stalder J-F, et al. The Emerging Role of Skin Microbiome in Atopic Dermatitis and Its Clinical Implication, Journal of Dermatological Treatment 2018.


13 Miajlovic H, et al. Effect of Filaggrin Breakdown Products on Growth of and Protein Expression


by Staphylococcus Aureus, Journal of Allergy and Clinical Immunology, 2010, 126: 1184- 1190


14 Korting H C. pH and Skin Care, Chapter 5, 31- 43: Skin pH and Cutaneaus Microflora, ABW Wissenschaftsverlag, 2007


15 Tang M L, et al. Probiotics and Prebiotics: Clinical Effects in Allergic Disease, Current Opinion in Pediatrics, 2010; 22(5): 626–634.


16 Kottner J. Transepidermal Water Loss in Young and Aged Healthy Humans: A Systematic Review and Meta-Analysis, Arch Dermatol Res, 2013; 305: 315-323.


17 Nachat-Kappes R. Probiotische Fraktionen – eine neue Lösung, die Hautgesundheit durch Stärkung der Barrierefunktion zu verbessern, indem die Hauthydratation erhöht und Entzündungen vorgebeugt wird, SÖFW Journal 2014; 140, 38-49


18 Guzman-Alonso M, Cortazar T M. Water content at different skin depths and the influence of moisturizing formulations, Household and Personal Care Today 2016; 11(1): 35-40.


19 https://dictionary.cambridge.org/de/ worterbuch/englisch/sebum


20 Sator P G, et al. Comparison of Epidermal Hydration and Skin Surface Lipids in Healthy Individuals and in Patients with Atopic Dermatitis, J Am Acad Dermatol 2003; 48: 352- 358;


21 Lanzendörfer G, Uhl C. How Effective is Your Anti-Acne Product, SPC 2018; 91 (12): 64-66


22 Goto E, et al. Tear Evaporation Dynamics in Normal Subjects and Subjects with Obstructive Meibomean Gland Dysfunction, Investigative Ophthalmology & Visual Science 2003; 44(2): 533-539


23 Fluhr J, et al. Full Body-Skin Mapping for Six Biophysical Parameters: baseline Values at 16 Anatomical Sites in 125 Human Subjects, Skin Pharmacology & Physiology 2012; 25: 25-33.


24 Dobrev H. Fluorescence Diagnostic in Patients with Acne, Photodermatol Photoimmunol Photomed 2010; 26(6): 285-289.


25 Wahlberg J E, Lindberg M. Assessment of Skin Blood Flow – An Overview, in Bioengineering of the Skin: Cutaneous Blood Flow an Erythema (Berardesca E, Ed.), 1995, 23-27.


26 Egelrud T. Desquamation in the Stratum Corneum, Acta Derm Venereol 2000; 80: Supp 208: 44-45


27 Rippke F, et al. The acidic milieu of the horny layer: New findings on the physiology and pathophysiology of skin pH. Am J Clin Dermatol 2002; 3: 261–272.


28 Strunk M, et al. Regeneration des physiologischen Hautoberflächen-pH nach Anwendung von Hautreinigungsmitteln, Poster, 13. Dermatologisches Alpenseminar Grainau, 2018


29 Korting HC, et al. Mikrobielle Flora und Geruch der menschlichen Haut. Hautarzt 1988; 39: 564–568.


30 Stenzaly-Achtert S, et al. Axillary pH and influence of deodorants. Skin Research & Technology 2000; 6: 87–91.


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