52 MARINE INGREDIENTS
19th century and was known for her elongated and delicate legs.
This particular species was selected due to its high content in omega 3, long chain EPA and DHA, carotenoids and sterols, to help the body fight against the oxidative stress of PM (particulate matter) and help to restore the skin barrier function. Several in vitro and in vivo studies were
performed at the mitochondrial level to demonstrate the major role of our Natura- Tec Marine CellShield AP to neutralise the activity of harmful pollutants (Fig 2).
In vitrostudies Anti-pollution effect: protection from indoor & urban dust Evaluation of the protective effects of our Natura-Tec Marine CellShield AP (now referred to as ‘the Pavlova lutheri active’) from 0.1% to 2% of use against pollution on Normal Human Epidermal Keratinocytes (NHEK) during 48 and 72 hours. More specifically, the effects of the compounds were evaluated on the viability of NHEK intoxicated with urban (Urban dust 1649b) or indoor (Indoor dust 2584) pollutant, following a standard MTT reduction protocol. The MTT (3-[4,5- dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay is based on the conversion of MTT into formazan crystals by living cells, which determines mitochondrial activity. The total mitochondrial activity is related to the number of viable cells. The experiment is repeated 3 times. The
measurement of the cell viability is carried out by photometry.
Result: Under the experimental conditions of this study, the intoxication of the NHEK with urban dust or with indoor dust induced a strong decrease of cell viability associated with a decrease of cell growth that was clearly observed already after 48 hours of incubation. Results after 72 hours add relevance to the test, subjecting keratinocytes to
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Recycle oils Energy CO2 Microalgae
minimal CO2 footprint
Waste Figure 1: The closed-circuit waste recycling system.
extreme conditions. The significance of the results obtained at 72 hours confirms the objectivity of this in vitro test.
After 48 hours and 72 hours incubation, we see that the Indoor dust has a negative impact on the cell viability: pollutants decrease cell viability respectively of 37% and 52% (Fig 3). When we use our Pavlova lutheri active, we see an immediate protective effect when tested at 0.5%, 1%, and 2% already after 48 hours of incubation.
After 48 hours and 72 hours incubation, we see that urban dust has a negative impact on cell viability: pollutants decrease cell viability respectively by 27% and 51% (Fig 4). When we use our Pavlova lutheri active, we see an immediate protective effect already with 0.2% after both 48 and 72 hours of incubation. And then from 0.5% of use, we achieve a maximum cell protection level and observe cell viability greater than in the non-intoxicated condition.
Environmental stress defence: maintains energetic metabolism Cytosolic ATP rate measurement. The bioluminescence method is based on measurements of adenosine triphosphate (ATP) which is the principal energy carrier in all living organisms and is involved in the functioning of most of metabolic process. This experiment is performed during 13 days in dermal
100% -52% 60% 48% 62% 66% 68% 70%
120 100 80 60 40 20 0
Control 0% 0.1% No Pollutants 0.2% 0.5% 1% 2%
Natura-Tec Marine CellShield AP concentration (%) Indoor Pollutants
Figure 3: Protection against indoor dust (72H). PERSONAL CARE EUROPE Figure 2: Mitochondria.
fibroblasts (NHDF) under environmental stress conditions induced by FCCP (carbonylcyanide p-trifluoromethoxy phenylhydrazone) from 0.1% to 0.2% of use of the Pavlova lutheri active (Fig 5). Result: FCCP induced a cytosolic ATP
rate reduction of 23%, indicating that chronic exposure to FCCP alters cellular ATP synthesis. The Pavlova lutheri active induced a significant increase of cytosolic ATP rate of +8% at 0.15% of use and up to +14% at 0.2% of use. This result shows that the treatment of cells by the Pavlova lutheri active modulates the decrease of ATP induced by the FCCP. The modulating effect is dose-dependent in
Ingredients
100% 114% 114% 114% -51% 81% 49% 97%
Control 0% 0.1% 0.2% 0.5% 1% No Pollutants
2%
Natura-Tec Marine CellShield AP concentration (%) Urban Pollutants
Figure 4: Protection against urban dust (72H). April 2019
Cell viability (%)
Cell viability (%)
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