36 SKIN CARE Control 1% Goji stem cell extract
10 8 6 4 2 0
Control CD34 Oct4 Nanog
Figure 2: Increase in stem cell marker expression in aged mesenchymal stem cells after treatment with goji stem cell extract.
eaten in Asia for their medicinal and nutritive properties. Today, goji berries are eaten all over the world, mostly in dried form, and there are various goji berry supplements on the market with the reported effects ranging from anti-ageing, immune boosting and energising to improved memory.
Producing plant stem cells To enable the large scale cultivation of plant stem cells the technology PhytoCellTec™ was used. This technology relies on the wound healing mechanism of a plant: part of a plant is wounded to induce the formation of callus cells. This healing tissue consists of dedifferentiated cells which are stem cells. Callus cells are harvested and cultivated in a suspension in a unique bioreactor. The goji stem cell active [PhytoCellTec™ Goji, INCI: Lycium Barbarum Callus Culture Extract (and) Isomalt (and) Lecithin (and) Aqua/Water] is based on a plant stem cell culture of a goji seedling and was tested for its effect on mesenchymal stem cells and exosome production.
300 2 1.5 1 0.5 0 Total protein quantification Enzyme activity quantification
Figure 3: Increase in exosome production by mesenchymal stem cells after treatment with goji stem cell extract.
Materials and methods Expression of stem cell markers in aged MSCs Adipose-derived human MSCs were grown for 14 passages to mimic the ageing process. These aged MSCs were then either treated or not (control) with 1% goji stem cell extract for 72 hours. At the end of incubation, the culture supernatants were discarded and cells were washed in phosphate buffered saline (PBS) solution and immediately frozen at -80°C. Total RNA was extracted from the cells and the expression of the stem cell markers CD34, Oct4 and Nanog was then assessed with RT- qPCR using the LightCycler System (Roche).
Quantification of exosome production by MSCs Dermal derived human MSCs were either treated or not (control) with 0.1% goji stem cell extract for a period of 24 hours. The exosomes that were released from the cells were isolated using EXOPrep kit (Hansabiomed) and quantified in two different ways: quantification of total protein amount and quantification of the activity of
Conditioned medium from untreated MSCs Conditioned medium from MSCs treated with 1% Goji stem cell extract
8 6 200 4 100 2 0 Control COL3A1 COL16A1 Elastin PLOD1 LOX
Figure 4: Gene expression of extracellular matrix genes by fibroblasts treated by conditioned medium from either untreated or goji stem cell extract treated mesenchymal stem cells.
PERSONAL CARE EUROPE 0
*p<0.05 versus untreated **p<0.01 versus untreated
Figure 5: Increase in epidermis + dermis density after 28 days treatment with 0.4% goji stem cell active.
April 2019 * **
Acetylcholinesterase, which is a known exosomal protein using the Pierce BCA Protein Assay kit (Thermo Fischer Scientific) and the Fluorocet exosome quantitation assay kit (System Biosciences), respectively.
Gene expression in fibroblasts after treatment with MSC-conditioned medium Human MSCs were seeded in 6-well plates and cultured in culture medium for 24 hours. The medium was then removed and replaced with culture medium containing or not (control conditioned medium) 1% goji stem cell extract and cells were incubated for 72 hours. All experimental conditions were performed in n=2. At the end of incubation, the supernatants were collected, and the replicates of each condition were pooled to be used for the treatment of fibroblasts (conditioned medium). Fibroblasts were previously seeded in 12-well plates and cultured in culture medium for 72 hours and then in assay medium for further 4 hours. Afterwards, for one series, medium was removed and
Placebo 0.4% Goji stem cell active * Control 1% Goji stem cell extract
*p<0.05 versus control
Change in gene expression compared to control (=100) in %
Fold change of stem cell markers normalized to control (=1)
Increase in skin density compared to untreated and initial conditions %
Increase in exsome levels normalized to control (=1)
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