140 ANTI-AGEING


Figure 7: Crow’s feet image with PRIMOS of a volunteer of 45 years old with an improvement in wrinkle volume of 32.0% after applying 3% Munapsys before (left) and after 7 days (right). Volunteer of the panel that applied 3% Munapsys and 5% benchmarks.


Double-blind assessment of anti- wrinkles efficacy Two panels of 18 female volunteers (35-55 years old) applied a cream with 3% the botulinum toxin-like peptide on half face twice daily for 14 days. A placebo or 5% benchmark was applied on the other half of the face. The benchmark was a blend of a pre-synaptic (Acetyl-Hexapeptide-8) and a post-synaptic (Dipeptide Diaminobutyroyl Benzylamide Diacetate) benchmarks. Roughness and wrinkles were assessed by PRIMOS and VISIA.


The botulinum toxin-like peptide always showed better results than placebo obtaining improvements of 7%, 10% and 7% in average roughness (Ra), average relief (Rz) and maximum relief height (Rt), after 14 days (Fig 4). In addition, the botulinum toxin-like peptide presented significant values in the three parameters of roughness and in maximum wrinkle depth at the end of the study (Fig 5). In addition, the group of volunteers that used 3% botulinum toxin-like peptide and 5% benchmark experienced a higher improvement in wrinkle reduction with a lower dose of the botulinum toxin-like peptide than with the benchmark (Fig 6 and Fig 7). This suggests a higher efficacy of the botulinum toxin-like peptide than of the benchmark combination. Moreover, in almost all the tested parameters there were more volunteers


responding to the cream with 3% botulinum toxin-like peptide than with the 5% benchmark. We can hypothesise that if there are more volunteers responding, it could be translated into more satisfied users.


Lifting sad smiles


A panel of female volunteers presenting a sad smile (drooping corners of the mouth), between 35 and 55 years old, applied a cream with 3% botulinum toxin-like peptide on the face for 28 days. The lifting of lip’s commissures was evaluated by clinical grading.


At the end of the study, 33% of volunteers presented an average lifting of commissures of 14%, showing a happier smile (Fig 8).


Conclusion


Munapsys demonstrated action on both pre- and post-synaptic pathways. It competed against Munc18-1 for its binding with syntaxin which is essential for the SNARE formation and it reduced the levels of ACh release by the pre-synaptic vesicles in the neuron. It also acted on AChR clustering, muscle membrane depolarization, calcium mobilisation and actomyosin cross-bridge and sliding. Finally, its efficacy as a wrinkle smoother was evaluated against a combination of benchmarks (covering both pathways) and proved to be similar or better at a lower


References 1 Zhou P, Pang ZP, Yang X, et al. Syntaxin-1 N-


peptide and Habc-domain perform distinct essential functions in synaptic vesicle fusion. EMBO J. 32(1):159-71, 2012.


2 Kavanagh DM, Smyth AM, Martin KJ, et al. A molecular toggle after exocytosis sequesters the presynaptic syntaxin1a molecules involved in prior vesicle fusion. Nat Commun. 5:5774, 2014.


3 Ma L, Rebane AA, Yang G, et al. Munc18-1- regulated stage-wise SNARE assembly underlying synaptic exocytosis. eLife. 4:e09580, 2015.


4 Huh KH, Fuhrer C. Clustering of nicotinic acetylcholine receptors: from the neuromuscular junction to interneuronal synapses. Mol Neurobiol. 25(1):79-112, 2002.


5 Banks GB, Fuhrer C, Adams ME, et al. The postsynaptic submembrane machinery at the neuromuscular junction: requirement for rapsyn and the utrophin/dystrophin-associated complex. J Neurocytol. 32(5-8):709-26, 2003.


6 Ruff RL. Endplate contributions to the safety factor for neuromuscular transmission. Muscle Nerve. 44(6):854-61, 2011.


7 Krans, J. L.The Sliding Filament Theory of Muscle Contraction. Nature Education 3(9):66, 2010.


concentration. In addition, Munapsys also presented good results in the lifting of sad smiles. We conclude that Munapsys could be


considered a real upgrade in the botulinum toxin-like ingredients field.


PC


T0 days


T28 days


Figure 8: Images of a volunteer of 51 years old that improved the dropping of the commissures by 16.7% after applying a cream with 3% Munapsys before (left) and after 28 days (right).


PERSONAL CARE EUROPE April 2019


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