120 ANTI-AGEING


Image 1: Human skin cells with the presently described technology (left) and those undergoing apoptosis (right) using holotomography


there were significant dose-related responses. As little as 0.0005% Epilobium fleischeri dry extract reduced senescent fibroblasts by 52%. This exciting result compares very well with the results for pure myricitrin, 25 μM of which reduced senescent fibroblasts by 45%.


Visualizing apoptosis in real time Holotomography is an innovative microscopy method that enables the clear imaging of living cells in their natural environment. Just as CT scans use differences in X-ray absorption to visualize the inside of the body, holotomography microscopy uses variations in the refractive index as a form of imaging contrast to view unaltered living cells and tissues.


This groundbreaking technique provides


detailed views of cellular structures and activities in real time and in their native contexts, overcoming the limitations of traditional microscopy approaches that rely on fixatives, dyes, and other interventions that in themselves, can change cell structures. Image 1 shows human skin cells with the presently described technology (left image) and those undergoing apoptosis (right image) using holotomography. The cell nucleus, with its nuclear material


and migrating mitochondria, can be clearly seen in the left image. The right image reveals similar detail, but in this image, one zombie cell has shrunk because it has entered apoptosis. In Image 2, this outstanding technology


is used to visualise the effects of Epilobium fleischeri extract on senescent cells in an in vitro model designed to mimic aged skin in real time. The aged skin model consisted of 50% normal fibroblasts and 50% senescent fibroblasts. At the 32-hour stage, a senescent cell is clearly visible. Just 30 minutes later, the same senescent cell can be seen to have entered apoptosis and is considerably smaller. After another 2.5 hours, a second adjacent senescent cell clearly shows signs of shrinking,


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Image 2: Holographic microscopy images as living human senescent fibroblast cells enter apoptosis after treatment with Epilobium Fleischeri Leaf/Stem Extract


and 30 minutes later, both senescent cells are in the later stages of apoptosis and removal. When holotomographic images from


experiments run over 48 hours are subject to AI-based analysis (Figure 7), the 100% normal fibroblasts treated with 0.0005% Epilobium fleischeri dry extract remain healthy. However, when the aged skin model


consisting of 50% normal fibroblasts and 50% senescent fibroblasts is treated with 0.0005% Epilobium fleischeri dry extract, a dramatic 200% increase in cell death is seen as


compared to the control. This demonstrates, in real time, that Epilobium fleischeri extract is very effective in reducing senescent cells, while leaving normal cells healthy.


Collagen boosting The expression of collagen I was assayed via an in vitro test using human fibroblast cells (50% normal and 50% senescent). When the fibroblast cells were pretreated with Epilobium fleischeri dry extract and compared to the untreated fibroblast cells (control), there was


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