SKIN PROTECTION 129 Control


Stratum corneum


Stratum


granulosum Figure 4: Inside-out diffusion through reconstructed human epidermis models. Presence of tracer is observed in green (cell nuclei are stained in blue).


ceramides, essential for the structure and impermeability of the barrier function.


Improved epidermal barrier functionality Two different dye penetration assays, which can be used as an in vitro indicator of transepidermal water loss (TEWL), were performed.


Stratum granulosum integrity Performance of the TJs in the granular layer of the epidermis was tested using biotin as a tracer molecule, which can pass through intercellular spaces but not through properly functioning TJs. Reconstructed human epidermis models were incubated for 24 hours with 50 μg/mL Acetyl Heptapeptide-4 and 0.02 mg/mL sodium dodecyl sulfate (SDS). Skin models treated with medium were used as a control and reconstructed human epidermis models incubated with 0.02 mg/mL SDS and medium were used as a positive control for barrier disruption. Then, a biotin tracer was added at the bottom of the epidermis models for 30 minutes to allow its inside- out diffusion. Presence of biotin tracer in the different layers was assessed by quantifying microscopy images. Biotin penetration through the stratum granulosum and into the stratum corneum was inhibited by 64.8% (p<0.0001) compared to the positive control, suggesting a reinforced barrier that can resist damage induced by SDS.


Stratum corneum integrity The intercellular lipids, that play a key role


Control


in the integrity of the stratum corneum, were evaluated by measuring the outside-in diffusion of the toluidine blue dye from the epidermal surface.


Reconstructed human epidermis models


were treated with 0.02 mg/mL SDS, in order to induce a disruption of the stratum corneum, in the presence or absence of 50 μg/mL Acetyl Heptapeptide-4. Models treated with medium were used as a control. After 24 hours, the dye was added on top of the epidermis models for 10 minutes and its diffusion across the epidermis was evaluated by microscopy imaging. A decreased penetration of tracer dye


through the stratum corneum was observed despite the presence of SDS, suggesting a strengthening of the barrier that prevents alterations by chemical aggressors.


Conclusion


Fensebiome peptide has shown to help strengthen vulnerable urban skin by reinforcing the skin’s microbial and physical barrier function on volunteers exposed to urban environments.


A metagenomics analysis on urban exposed volunteers showed that the ingredient helped increase microbial diversity, favored the presence of beneficial bacteria on the skin and modulated bacterial functional pathways with potential benefits for the skin. Other clinical studies on female urban subjects showed to be efficient in reinforcing cell cohesion and in reducing TEWL levels after damage for a protective effect of the skin barrier. When evaluated on skin models, it helped improve the functionality of the epidermal


SDS


barrier through a reduced permeability. In vitro, the peptide helped to promote the adhesion of beneficial bacteria to keratinocytes and to stimulate the cutaneous immune response, through pathogen recognition pathways, to keep the skin ready in case of any potential invasion. An enhancement in tight junction genes and long-chain ceramides were also obtained, contributing to a well-preserved skin barrier. Fensebiome peptide represents a way to


reconnect with our origins, since it helps increase cutaneous bacterial diversity and favors the beneficial bacteria on the skin, which is a marker of the healthy and protected skin of populations in closer contact with nature.


References 1 Clemente J, Pehrsson EC, Blasser MJ, et al.


The microbiome of uncontacted Amerindians. Sci. Adv. 2015; 1(3) :e1500183


2 Chiller K, Selkin B, Murakawa G. Skin microflora and bacterial infections of the skin. Journal of Investigative Dermatology Symposium Proceedings 2001; 6(3):170-174, 2001.


3 Sanford J, Gallo. Microbial Symbiosis with the innate immune defense system of the skin. J Invest Dermatol. 2011; 131(10): 1974–1980.


4 Von Herzen L, Hanski I, Haahtela T. Natural immunity. EMBO reports 2011; 12(11):1089-1093


5 Zaniboni MC, Samorano LP, Orfali RL, Aoki V. Skin barrier in atopic dermatitis: beyond filaggrin. An Bras Dermatol. 2016; 91(4):472-8


FENSEBIOME™ is owned by The Lubrizol Corporation or its affiliates. © 2018 The Lubrizol Corporation. All Rights Reserved.


SDS + Acetyl Heptapeptide-4 SDS SDS + Acetyl Heptapeptide-4


Stratum corneum


Figure 5: Outside-in diffusion of the toluidine blue dye into the stratum corneum under different conditions. April 2018 PERSONAL CARE EUROPE


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