SKIN PROTECTION 123 Active with 1% IBR-UrBioTect Placebo 10.0 N.S 5.0 0.5 0.0 D14 -0.5 -.10.0 -8.8 * -0.3 D28 7.2
14.0 12.0 10.0 8.0 6.0 4.0 2.0 0.0
12.7 N.S Active with 1% IBR-UrBioTect Placebo 12.5 *
3.1 1.9
D14 Figure 2:Wrinkle count variation.
meaningful increase in ITA (up to 25% at D28), with statistical significance vs. study D0 and vs. the placebo. The effect is shown to progressively build up over the course of the study.
Helps repair wrinkles in a smoking environment Visia-CA image analysis data show that daily application of the active product results in a reduction in wrinkle counts after 28 days, statistically significant vs. D0 and vs. the placebo.
Improves skin elasticity in a smoking environment
Cutometer data shows that daily application of the active product results in a meaningful improvement in skin elasticity after 28 days, statistically significant vs. D0 and vs. the placebo. Here again, the effect is shown to build up over the course of the study (the improvement is already visible but has not yet acquired statistical significance, at D14).
0.0 -0.5 -0.10 -15.0 -20.0 -20.2 -25.0 -30.0 Figure 4: TEWL malar evolution. April 2018 Active with 1% IBR-UrBioTect Placebo -17.8 * * -7.8 -7.3 Figure 3: Elasticity (R2) variation periorbital.
Enhances skin barrier function in a smoking environment Trans Epidermal Water Loss (TEWL) data shows that daily application of the active product results in a rapid and meaningful improvement in skin barrier function, as expressed by a reduction in trans- epidermal water loss after 14 days, with statistical significance vs. D0 and vs. the placebo. The effect is maintained at D28.
Illustrative images
The illustrative photographs in Figure 5 demonstrate the reduction in the appearance of wrinkles and the improvement in skin tone resulting from daily application of the active product.
Protects from heavy metals: chelation (Rohman et al.2010) Following on the clinical results described above, and in order to begin providing mechanistic explanations for the effects observed, the chelating power of Inula
helenium extract, was evaluated according to the method described in Rohman et al. (2010). In this method, the ferrous ion is used as a model for heavy metals. The concentration of the ferrous iron-Ferrozine complex is measured by spectrophotometry (absorbance at 562 nm). Addition of a chelator, capturing iron away from said complex, results in a drop in the measured absorption. EDTA was used as positive control and comparison point. Chelating power is expressed as an IC50 value (mg/ml), being the concentration of tested product at which 50% of available iron was chelated. In this model, an Inula helenium extract was shown to have an IC50 of 0.54 mg/mL (expressed on a dry weight basis), indicating that the product possesses chelating power, ca. 1 order of magnitude below that of reference chelator EDTA on a dry weight basis, measured at 0.038 mg/mL.
Detoxifying and pre-biotic benefits Detoxifying and some pre-biotic benefits were shown with Dunaliella salina cell culture as a model for evaluating the anti- pollution effect. To further expand the scope of our investigations into the detoxifying potential of Inula helenium extract, we used the microalgae, Dunaliella salina as an animal- and human-free model to demonstrate the product’s efficacy. The unicellular alga Dunaliella salina was selected as a sensitive, reliable, and cost effective model system for evaluating the protective effects of an Inula helenium extract against exogenous toxins. Algae, and micro algae specifically, are commonly used as a sensitive model system to evaluate water quality. Dunaliella cultures were incubated with Inula helenium extract at concentrations of
PERSONAL CARE EUROPE
D28
% change (%) from D0
% change (%) from D0
% change (%) from D0
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92 |
Page 93 |
Page 94 |
Page 95 |
Page 96 |
Page 97 |
Page 98 |
Page 99 |
Page 100 |
Page 101 |
Page 102 |
Page 103 |
Page 104 |
Page 105 |
Page 106 |
Page 107 |
Page 108 |
Page 109 |
Page 110 |
Page 111 |
Page 112 |
Page 113 |
Page 114 |
Page 115 |
Page 116 |
Page 117 |
Page 118 |
Page 119 |
Page 120 |
Page 121 |
Page 122 |
Page 123 |
Page 124 |
Page 125 |
Page 126 |
Page 127 |
Page 128 |
Page 129 |
Page 130 |
Page 131 |
Page 132 |
Page 133 |
Page 134 |
Page 135 |
Page 136 |
Page 137 |
Page 138 |
Page 139 |
Page 140 |
Page 141 |
Page 142 |
Page 143 |
Page 144 |
Page 145 |
Page 146 |
Page 147 |
Page 148 |
Page 149 |
Page 150 |
Page 151 |
Page 152 |
Page 153 |
Page 154 |
Page 155 |
Page 156 |
Page 157 |
Page 158 |
Page 159 |
Page 160 |
Page 161 |
Page 162 |
Page 163 |
Page 164 |
Page 165 |
Page 166 |
Page 167 |
Page 168 |
Page 169 |
Page 170 |
Page 171 |
Page 172 |
Page 173 |
Page 174 |
Page 175 |
Page 176 |
Page 177 |
Page 178 |
Page 179 |
Page 180 |
Page 181 |
Page 182 |
Page 183 |
Page 184
