124 SKIN PROTECTION
0% (control), 1% and 2%, for 4 days. After incubation, the cell density (cells/ml) of the culture was evaluated using a digital camera at time point 0 (T0). Selected toxins (respectively: methanol at 60,000 ppm, gasoline at 500 ppm and formaldehyde at 25 ppm) were added to the cultures at T0 and the cultures were grown in the presence of Inula helenium extract for another 48 hours. After 48 hours, the cell density (cells/ml) of the cultures was evaluated. Culture growth was calculated as the ratio between cell density at 48 hours and cell density at T0. These results also show that the
presence of Inula helenium extract at least partially rescues the Dunaliella salina cell culture from the toxic effects of each of the three toxins introduced. At 1% Inula helenium extract has a prebiotic effect which increases the algae cells growth by about 21%, 2% Inula helenium extract enhances algal growth by about 30%.
Protecting from blue light-HEV In order to explore more angles of protection from multiple types of pollutants by Inula helenium extract, a study with blue Light (HEV) was conducted on skin cells. We demonstrated that Inula helenium extract can efficiently protect skin cells from HEV induced ROS production, adding significant protective benefits against environmental pollution.
Conclusion We propose here a natural solution for protecting and detoxifying skin against and ever-increasingly polluted urban environment. As we have shown above: IBR-UrBioTect was able to repair and
reverse the effects of pollution in a clinical study in a smoking environment (cigarette smoke). It could significantly inhibit blue light-induced ROS production preventing accumulation of ROS in skin cells which may result in signs of photoageing and hyperpigmentation. It was also shown to be a metal chelator, possibly helping to neutralise heavy metal pollutants which
D0 D28
Figure 5: Illustrative images w/o 1% 2%
160% 140% 120% 100% 80% 60% 40% 20% 0%
Non toxin Methanol IBR-UrBioTect Figure 6: Detoxification effect (Dunaliella salina culture).
cause accumulated damage to the skin. IBR-UrBioTect could mitigate the toxicity of pollutants in aquaculture and it also showed pre-biotic benefits in aquaculture. IBR-UrBioTect is a natural and innovative solution helping to relieve and repair the effects of pollution on skin and the
1.5
appearance of early signs of ageing. The product promotes a lighter skin tone in a smoking environment, reduces wrinkle count, and improves skin elasticity and barrier function. More studies are under way to further demonstrate this and other activities of IBR-UrBioTect.
PC Gasoline Formaldehyde
1
0.5
0 Control
Figure 7: Dunaliella culture contaminated with gasoline. (left: 0% Inula helenium extract; centre: with 1% Inula helenium extract; right: with 2% Inula helenium extract).
PERSONAL CARE EUROPE
Control + HEV
0.125% IBR-UBT + HEV
0.25% IBR-UBT + HEV
0.5%
IBR-UBT + HEV
Figure 8: IBR-UrBioTect significantly inhibits HEV-induced ROS production in human keratinocytes (HaCaT).
April 2018
ROS normalized to irradiated control
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