94 ANTI-AGEING
in vitro has been confirmed by the results of the in vivo tests. A group of 30 female participants aged 40-50 years took part in a double-blind, randomized and placebo-controlled clinical study. All of them were presenting crow’s feet wrinkles and pigmentation spots. The new ingredient was applied at 1%
formulated in a cream. Skin elasticity was measured at the beginning of the study and after 28 and 56 days of application. Several clinical parameters which increase when skin elasticity increases were registered by a Cutometer. The LC postbiotic generated a significant
increase of skin elasticity with an improvement of up to 10% compared to the baseline and placebo in all of these parameters (see Figure 7). After 56 days of treatment, the new ingredient significantly decreased crow’s feet average compared to the placebo by almost 6.6%. Another effect could be verified: the LC postbiotic decreased the melanin index of age spots compared to the placebo by almost 6.5% – an indication of its skin-lightening capacity.
New biotic ingredients support healthful skin ageing Probiolift and Postbiolift are the first cosmetic ingredients which use Lactobacillus crispatus, a bacterium found naturally in the microbiome of our skin. BASF discovered the role of Lactobacillus crispatus as part of a study on the skin microbiome, especially comparing younger and older skin microbiota. With the LC probiotic, a particular challenge could be solved: incorporating living skin bacteria into cosmetic formulations and keeping them active. In view of the research findings, the two new cosmetic ingredients from the same fermentation process of Lactobacillus crispatus unlock a still largely untapped potential in the biotic beauty sector. They can thus be seen as game-changers in
the rapidly growing market for natural, healthy- ageing cosmetics.
Elastin synthesis stimulation
160 140 120 100 80 60 40 20 0
Untreated control
The trademarks symbolized with a ® or ™ are either property of or licensed to BASF group and registered and/or applied for registration in relevant countries. Other product names and trademarks mentioned may belong to third parties.
PC
References 1. Dimitriu PA, Iker B, Malik K, Leung H, Mohn WW, Hillebrand GG. New Insights into the Intrinsic and Extrinsic Factors That Shape the Human Skin Microbiome. mBio. 2019. 10(4):e00839-19
2. Shibagaki N, Suda W, Clavaud C, Bastien P, Takayasu L, Iioka E, Kurokawa R, Yamashita N, Hattori Y, Shindo C, Breton L, Hattori M. Ageing-related changes in the diversity of women’s skin microbiomes associated with oral bacteria. Sci Rep. 2017;7: 1-10
3. Pan M, Hidalgo-Cantabrana C, Goh YJ, Sanozky-Dawes R, Barrangou R. Comparative Analysis of Lactobacillus gasseri and Lactobacillus crispatus Isolated From Human Urogenital and Gastrointestinal Tracts. Front Microbiol. 2020.10:3146
Biomechanical properties R2 R5 - parameters
(***)/PI
14% 12% 10% 8% 6% 4% 2% 0%
9.5% (*)/PI 3.3%
(***) /D0
4.4%
D28 R2 Gross elasticity
D56 D28
12.9% (***)
/D0
5.9% (**)
+10.1%
/D0 (*) /D0
5.8%
(**) /D0
D56 R5 Immediate elasticity (**)/PI
(***) /D0
11.7%
14% 12% 10% 8% 6% 4% 2% 0%
(**) +28%
Statistics: Mean + SD n=6 Student t test (**) p<0.01
LC Probiotic 0.05% Figure 6: Effect of the LC postbiotic on elastin synthesis by fibroblasts
4. Abruzzo A, Parolin C, Corazza E, Giordani B, Pio di Cagno M, Cerchiara T, Bigucci F, Vitali B, Luppi B. Influence of Lactobacillus Biosurfactants on Skin Permeation of Hydrocortisone. Pharmaceutics. 2021;13:820
5. Lebeer S, Vanderleyden J, De Keersmaecker S CJ. Genes and Molecules of Lactobacilli Supporting Probiotic Action. Microbiology and Molecular Biology Reviews. 2008;72:728-764
6. Duar RM, Lin XB, Zheng J, Martino ME, Grenier T, Perez-Munoz ME, Leulier F, Ganzle M, Walter J. Lifestyles in transition: evolution and natural history of the genus Lactobacillus, FEMS Microbiology Reviews. 2017;41:27-48
7. Microbes in Allergy and Autoimmunity Related to the Skin (MAARS) Final Report Summary FP7-HEALTH, https://cordis.
europa.eu/project/id/261366/reporting
8. Zhang S, Duan E. Fighting against Skin Ageing: The Way from Bench to Bedside. Cell Transplantation. 2018;27(5): 729–738
9. Lee A-Y. Skin Pigmentation Abnormalities and Their Possible Relationship with Skin Ageing. Int. J. Mol. Sci. 2021;22: 3727
Biomechanical properties Q1 - parameters
(***)/PI
(***)/D0 12.5%
(***)/D0 4.7%
2.9% D28 Total Recovery
■ Placebo ■ LC Postbiotic at 1%
Figure 7: Effect of the LC postbiotic on skin elasticity measured by a Cutometer after application for 56 days PERSONAL CARE April 2023
www.personalcaremagazine.com
Statistics: Percentage on (*) p<0.05 30 volunteers Wilcoxon test
(**) p<0.01 (***) p<0.001 D56 9.8%
% vs D0
Elastin synthesis (%)
% vs D0
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