EMS MATERIALS SCIENCE & METROLOGY CATALOG 2019–20 EDITION TOOLS
MicroTools for Crystallography (continued) 2/ Individual MicroMount™
MicroMounts™ (continued)
Each box contains 20 MicroMount™ with your choice of size and pin length. In general, we recommend ordering our 19mm or SPINE length pins. If you like copper post style goniometer bases and have been using 10 or 11mm pins with them, consider our B3 copper post base, which accepts full 19mm length pins.
Catalog Number Size Qty. Pin 11mm Pin 19mm Pin 25mm SPINE
10µm Box of 20 60115-11 60115-19 60115-25 60115-S 20µm Box of 20 60116-11 60116-19 60116-25 60116-S 30µm Box of 20 60117-11 60117-19 60117-25 60117-S 50µm Box of 20 60118-11 60118-19 60118-25 60118-S 75µm Box of 20 60119-11 60119-19 60119-25 60119-S 100µm Box of 20 60120-11 60120-19 60120-25 60120-S 150µm Box of 20 60121-11 60121-19 60121-25 60121-S 200µm Box of 20 60122-11 60122-19 60122-25 60122-S 300µm Box of 20 60123-11 60123-19 60123-25 60123-S 400µm Box of 20 60124-11 60124-19 60124-25 60124-S 500µm Box of 20 60125-11 60125-19 60125-25 60125-S 600µm Box of 20 60126-11 60126-19 60126-25 60126-S
Liquid Wicks
These paper wicks offer a great deal of absorbency with a small tip that foam-tipped or cotton-tipped applicators can not duplicate. These wicks are available sizes: 15, extra fine (XF), fine (F), and Medium (M).
Size 15 wicks fit the wicking aperture of MicroMounts, and are ideal for removing residual liquid from your crystal and mounts. Size Medium (M) and Fine (F) wicks are ideal for cleaning MicroMount
The thick end of these wicks fit into standard 0.7mm mechanical pencil. ORDERING INFORMATION
Each box contains 300, 28mm long high quality, tapered paper wicks.
Order size 15 and Extra Fine (XF) for removing liquid formed around your crystal.
Order sizes Fine (F) and Medium (M) wicks for cleaning MicroMounts.
How it works First, we’ve replaced the
standard 10µm wall glass capillaries with ultra-thin-wall transparent polyester tubing that produces 60% less background scatter. Second, we use a tubing diameter that’s much larger than your crystal and MicroMount, and a
base that both holds the MicroMount and captures the tubing.
To prepare the sample, just inject stabilizing liquid down into the tube toward its sealed end, using a gel-loading pipette-tip. Mount your crystal on MicroMount and insert MicroMount into the base. Thirdly, pull the tubing down over the crystal and mount onto the base.
No more cutting, crushing, and breaking. No more cracking and shattering crystals on sharp capillary edges. No more swishing crystals back and forth to position them. No more fiddling with wicks. No more wax. No more alignment problem caused by capillary/liquid lensing.
Once you have collected your room temperature frames, just pop off the tubing, plunge the MicroMount in your choice of liquid cryogen, and you’re ready to collect a low-temperature data set. Or else, soak your crystal in the next solution, pop the plastic tubing back on, and repeat until you’re checked out each solution or step in your protocol.
Cat No. Description 60160-15 Size 15 Liquid Wicks
60160-XF Size Extra-Fine Liquid Wicks 60160-F Size Fine Liquid Wicks 60160-M Size Medium Liquid Wicks
Qty 300 300 300 300
The only disadvantage is that polyester is somewhat gas permeable. You will want to keep a good sized plug of liquid in the tube and to measure your crystals within a day of mounting to make sure they do not dehydrate. If you want to store the crystals for longer, you can replace the polyester tube with any 2mm ID glass or thick wall plastic tubing.
MicroRT™ Room Temperature Mounting System MicroRT™ is the answer for high-throughput room temperature diffraction screening and data collection. Go from crystal in a drop to crystal in the x- ray beam at room temperature in 2 minutes with 99% chance of success.
No more dealing with inadequate low temperature diffraction quality, painful glass capillary mounting methods, or difficult retrieval of crystals for low- temperature data collection.
Room temperature measurements are essential to determine whether the cause is poor as –grown crystal quality, damage caused by ligand and/or cryoprotectant soaks, and/or damage caused by the flask cooling process itself.
Our patent-pending MicroRT™ system makes collecting data at room temperature as easy as at low temperatures, and allows you to collect room and low temperature data from the same crystal.
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