EMS MATERIALS SCIENCE & METROLOGY CATALOG 2019–20 EDITION
TOOLS MicroTools for Crystallography (continued)
MicroMounts™ MicroMounts™
are the highest performance tools for manipulating and mounting protein crystals, virus crystals, and small molecule/inorganic crystals, as well as all other small, fragile samples. They are ideal for both conventional and high-throughput X-ray crystallography applications.
MicroMounts™ consist of:
A thin (7.5µm thick) micro-fabricated polyimide film, attached to a solid non-magnetic stainless steel pin. Film is wrapped around the steel pin; provides excellent stiffness and convenient scoop-like action in retrieving and handling crystals.
FEATURES Easy manipulating and mounting of crystals from 700µm to less than 5µm.
Rigid yet thin construction Convenient scoop-like design Gold hue – good mount crystal contrast Ideal for use at microfocus beamlines Complete optimization of cryoprotection and flash cooling Completely non-magnetic Low Thermal mass – rapid cooling Crystal aperture can be matched to crystal size and shape Easy removal of excess liquid Fully compatible with existing crystal mounting hardware
Film Pattern: Crystal Aperture sizes from 10 to 600µm, with minimal width around the aperture to minimize background scatter in all orientations.
Wicking Hole connected via drainage channel D to the crystal aperture. Hole size is compatible with size 15 or XF paper wicks. This ‘fountain-pen’- like design, allows easier removal of excess liquid, without touching the crystals with the wick.
Alignment Cross located a fixed distance from the center of the crystal aperture. Allow easy automated alignment of the crystal aperture
Crystal Aperture Size Code around the alignment cross, allows automated recognition of Mount design, aperture, and crystal size, which determines boundaries of aperture (to be avoided), required alignment tolerance, ad area of crystal available to be scanned for optimum
ORDERING INFORMATION 1/ Assortment Boxes:
We recommend initially ordering of any assortment apertures listed below, so you can match the amount aperture to the size of your crystal. Then you order the size you use the most. You can choose pin lengths from 11, 19, 25, or SPINE. These pin pins can be cut to a desired length (using cutter pliers) or bent to your orientation.
diffraction. Initial beam size can then be automatically selected. Aperture Size in µm can be read directly off the mount.
Orientation Mark allows the front and back of the mount to be automatically distinguished. Pin:
Solid 316 non-magnetic stainless steel pins. Pin is available in four standard lengths: 11 mm, 19 mm, 25 mm, and SPINE. The 11, 19, and 25 mm pin lengths, give pin base-to-crystal center distance compatible to those of nylon loops mounted on 10, 18 and 24 mm pins, respectively.
This solid pin will not suck up crystal and liquid. Pin is beveled at film end, allows good visibility of the crystals for wide range of pin orientation during crystal retrieval. Pin can be cut (with cutter pliers) to any desiredlength, or can be bent to place crystal in desired orientation. Pin is fitted to all standard bases
SPECIFICATIONS
Pins Length
Diameter Material Pin End
Crystal Holding Mount Material
Crystal Aperture Sizes Thickness
Distance from center of E to center of A
Distance from pin base
Width of Wicking Hole Length Tolerance
11, 19, 25 mm and SPINE lengths, marked in 2 mm intervals. 0.025” (0.64 mm) diameter
Solid 316 non-magnetic stainless steel Beveled at 30º angle at each end
Polyimide
10, 20, 30, 50, 75, 100, 150, 200, 300, 400, 500 and 600µm 7.5, 10 and 12.5 µm 870 µm
12.1 mm (for 11 mm pins),
to center of crystal aperture 20.1 mm (for 19 mm pins), 26.1 mm (for 25 mm pins), 18.5 mm (for SPINE length pins) 250 µm (~ size 15 paper wicks)
0.5 mm for 11, 19, and 25 mm pins, 0.3 mm for SPINE pins
Small (A1),
includes: 10, 20, 30 and 50 µm apertures
Description
MicroMount™ Assortment Box A1 MicroMount™ Assortment Box A2 MicroMount™ Assortment Box A3
114
Pin 11mm 60110-11 60111-11 60112-11
Medium (A2), includes: 75, 100, 150, and 200 µm apertures
Catalog Number
Pin 19mm 60110-19 60111-19 60112-19
Pin 25mm 60110-25 60111-25 60112-25
SPINE
60110-S 60111-S 60112-S
Large (A3),
includes: 300, 400, 500, and 600 µm apertures
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