EMS MATERIALS SCIENCE & METROLOGY CATALOG 2019–20 EDITION TOOLS
MicroTools for Crystallography (continued) MicroMesh™ Mounts
MicroMesh™ Mounts are the tool of choice for microcrystal crystallography and diffraction experiments, especially at micro-focus beam-lines. They have been use in de novo protein structure determination from crystals as small as 5µm. They are excellent for rods shaped crystals, and are superior to mount with elliptical apertures, because the mesh provides continuous, gentle support for rods of all sizes.
LV CryoOil™ Oils are widely used in crystallography – as
cryoprotectants when samples are flash cooled, and as barriers to the dehydration, solvent evaporation, and oxidation, that may occur in air near and above room temperature. The ice ring seen in the diffraction pattern of flash cooled protein crystals arise primarily from
crystallization of the aqueous solution surrounding the crystal,
MicroMesh™ produce the smallest background scatter of any commercial mount. Their sieve-like action allows easy retrieval of sub 30µm crystals.
We offer five different sizes MicroMesh™. All are 10µm thick, with curved around tip, mounted on non-magnetic stainless steel pin as the same way as MicroMount™, producing scoop like action.
Three of 400 µm diameter mesh area with 10, 25, and 50 µm openings One of 700 µm diameter with mesh area with 25 µm openings.
These four are available as single-size boxes, or in an assortment. Multiple small crystals can be retrieved and mounted together, spread out over the mesh. The diffraction properties of each crystals can be separately interrogated using a small (<100 µm) X-ray beam, so crystal heterogeneity and maximum diffraction quality yielded by a given drop’s conditions can be quickly determined. This is useful in early crystallization trials when showers of crystals and precipitate are obtained.
When a drop contains many crystals, two or more crystallographic forms may be present which can differ dramatically in the quality of their diffraction. The regular grid of each MicroMesh makes it easy to match a diffraction pattern with a crystal and habit. Minimal crystals can be used.
Excess liquid can be removed using size #15 liquid wicks. Mounts can be cleaned using Medium or Fine liquid wicks.
The fifth MicroMesh™ Mount has an 80 µm diameter mesh area with 15µm openings.
This small head is designed to fit entirely within the 80µm and larger X-ray beams typical of standard synchrotron beam-lines and of focused laboratory sources. Consequently, you do not have to see your tiny crystal to align it, just center the 80 µm head in the beam, and your crystal will be within it.
The smaller head also makes it easier to mount a single crystal, and produces less background scatter when the beam is aligned along the plane of the film.
ORDERING INFORMATION Each box contains 20 MicroMesh™ Mounts
Assortment box contain 5 each of 400/10, 400/25, 400/50, 700/25, and 800/15 MicroMesh mounts, each attached to nonmagnetic stainless steel pin. Pins are marked in 2 mm intervals, and can be cut or to your desired length.
Catalog Number Description
Assortment Box, 20 Micromesh Mount (20) 400µm Diameter, 10µm openings (20) 400µm Diameter, 25µm openings (20) 400µm Diameter, 50µm openings (20) 700µm Diameter, 25µm openings (20) 80µm Diameter, 15µm openings
Pin 11mm Pin 19mm Pin 25mm 60150-11 60150-19 60151-11 60151-19 60152-11 60152-19 60153-11 60153-19 60154-11 60154-19 60155-11 60155-19
60150-25 60151-25 60152-25 60153-25 60154-25 60155-25
SPINE
60150-S 60151-S 60152-S 60153-S 60154-S 60155-S
NOTE: to specify the pin length, substitute xx with the Pin Length: 11mm, 19 mm, 25 mm, or SP for SPINE). Example: 60150-11 for pin length 11 mm; 60153-SP for SPINE pin length.
not of the internal solvent. Oils can displace and replace this surrounding solution with little risk of damage due to osmotic shock.
Substantial dehydration-induced damage can easily occur between removed from a drop and flash cooling. Dehydration can be minimized by first transferring to an oil drop (using MicroSieve™, in MicroTool kit 1 and MicroTool Kit 2) before mounting the oil coated crystal on a MicroMount or Micromesh.
However, surrounding oil scatters X-rays, reducing diffraction signal-to-noise, and contributes thermal mass that reduces crystal cooling rates and increases the need for penetrating cryoprotectants. These problems are especially acute for the smallest crystals. When mineral oil, Paratone Oil and other high viscosity oils are used, the volume of surrounding oil can exceed the crystal volume by a factor of 10 or more., and can contribute excess drag that increases sample motion in a cryostream. For larger rod or plate shaped crystals, the surrounding oil exerts stresses when it freezes that can cause crystal cracking and mosaic broadening.
LV CryoOil™ Is the best solution to your oil needs. It has the lowest viscosity of any available perfluoropolyether oil (1/10 that of Paratone oil and compatible to that of vegetable oil) and very low surface tension (less than 1/3 that of water). Consequently, a dip in this oil followed by gentle tapping to shake off excess can yield protective oil films on your crystals of as little as 10 µm thick.
LV CryoOil™ has extremely low vapor pressure, excellent chemical inert and excellent thermal stability.
LV CryoOil™ comes in each vial 1.5 ml, enough to protect hundreds of crystals.
Cat No 60166
Description LV CryoOil™
Qty. 1.5 ml
113
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