COVER STORY: ASHLAND CTR ■ 0.1% Iris extract ■
500 450 400 350 300 250 200 150 100 50 0
P7 P12 young cells 1.19*** years younger -0.78
fast ageing cells control
-54% *
D28-D0 0.41
slow aging cells Iris extract
-80% ***
0.68*** years younger -0.44
D14-D0 0.24 P24 ageing cells
Figure 3: SA-β-galactosidase activity in fibroblasts treated with Iris pallida extract every day from P7 to P30, ***: highly significant, *: significant with Student t-test, mean +/- sem, n=6
observed by SA-β-galactosidase activity staining. A clear delay in the senescence appearance was observed in the treated cells compared to the control where the senescence appeared sooner and increased faster (figure 3). Treated cells can thus be define as « slow agers » compared to non- treated cells.
Clinical study design To evaluate, in vivo, the effect of Iris pallida extract on nighttime skin regeneration to reverse the signs of aging, a double-blind clinical study was carried out on 35 volunteers (35-76 years- mean age: 51.31) applying the Iris Pallida extract formulations following a night- routine. All evening, for 1 month, volunteers applied, first, a serum following by a cream containing 1% Iris Pallida or not. The morning, all volunteers applied a placebo cream to ensure all participants were using the same product. The clinical study was conducted in May-June to mimic accelerated aging induced by increased sun and temperature exposure during this time.
D0
Placebo treated group D28
P30 ∆ AGE (DX-D0) Placebo treated group ■ 1% Iris treated group ■
Figure 4: Evaluation of skin perceived age by A.I facial scanner, *: significant; ***: highly significant, with Student’s t-test or Mann Whiteney test depending on whether the data followed a normal distribution at determined times
Reverse perceived skin age Perceived age was assessed using the artificial intelligence-based facial analysis software SkinAnalys.I.A. This software analyzes skin directly from photographs to estimate perceived age. The AI algorithm is trained to determine the similarities between the photo taken at the time and photos annotated by dermatologists according to seven skin parameters (wrinkles, hydration, firmness, pores, evenness, pigmentation spots, and eye contour). Thus, the perceived age estimated is a reliable measure, based on several parameters known to influence perceived age. After one month of use, volunteers using
formulations based on Iris pallida extract appeared, on average, 1.2 years younger, and this perceived rejuvenation could reach 4 years. Conversely, volunteers in the placebo group appeared 5 months older, likely due to summer conditions (figure 4). This result demonstrates that perceived rejuvenation can be mitigated by nighttime skin repair.
D0
1% Iris extract treated group D28
Revive the visible signs of youth In addition to skin perceived age, skin wrinkling, one of the main signs of age on Caucasian skin, was measured by combination of fringe projection and stereometry technologies with the AEAVA-HE* system (Eotech*). After one month of applications, with the
biofunctional containing cream, softens the appearance of wrinkles of the eye contour, significantly decreased in volume, area, and circumference (Figure 5). Besides, other visible signs of youth as skin luminosity, redness and hydration were assessed and here again, the group applicating 1% Iris pallida formulations showed statistically significant improvement compared to placebo for all evaluated parameters. In summary, in one month, the 1% Iris pallida
extract, applied following a nighttime skincare routine, diminished the appearance of wrinkles and improved skin tone, thus reducing the perceived age of the skin by 1.2 years compared to the placebo group.
Vol#7; 64 years-old-Caucasian skin Wrinkle’s volume
0.2 0.1 0.0
-0.1 -0.2 -0.3
ns D14-D0 -208%* D28-D0
15 10 5 0
-5
-10 -15
D14-D0 D28-D0 ns -163%* Wrinkle’s circumference
Placebo treated group ■ 1% Iris pallida treated group ■ Wrinkle’s area
3 2 1
0 -1 -2 -3
ns D14-D0 -213%* D28-D0
Figure 5: Evaluation of skin perceived age by A.I facial scanner, ns: not significant, *: significant; with Student’s t-test or Mann Whiteney test depending on whether the data followed a normal distribution at determined times
www.personalcaremagazine.com
Ashland 500 Hercules Road, Wilmington, DE 19808, USA
Ashland.com/personalcare
April 2026 PERSONAL CARE MAGAZINE
Conclusion Mitobiology™ introduces a new longevity paradigm by restoring cellular intelligence through humanin-driven mitorestoration. Ashland’s Iris pallida extract helps the skin supports nighttime mitochondrial repair, delivering visible clinical improvements and reduced perceived age. This science led approach enables next generation nighttime longevity skincare and anti-aging formulations with credible, repair and long term skin health benefits.
References References to this article are available at:
Ashland.com/eternight-references
ADVERTISEMENT FEATURE 7
PCM
∆ volume (mm3
)
Total blue area / cell
∆ circumference (mm3
)
∆ area (mm3
)
more senescence
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92 |
Page 93 |
Page 94 |
Page 95 |
Page 96 |
Page 97 |
Page 98 |
Page 99 |
Page 100 |
Page 101 |
Page 102 |
Page 103 |
Page 104 |
Page 105 |
Page 106 |
Page 107 |
Page 108 |
Page 109 |
Page 110 |
Page 111 |
Page 112 |
Page 113 |
Page 114 |
Page 115 |
Page 116 |
Page 117 |
Page 118 |
Page 119 |
Page 120 |
Page 121 |
Page 122 |
Page 123 |
Page 124 |
Page 125 |
Page 126 |
Page 127 |
Page 128 |
Page 129 |
Page 130 |
Page 131 |
Page 132 |
Page 133 |
Page 134 |
Page 135 |
Page 136 |
Page 137 |
Page 138 |
Page 139 |
Page 140 |
Page 141 |
Page 142 |
Page 143 |
Page 144 |
Page 145 |
Page 146 |
Page 147 |
Page 148 |
Page 149 |
Page 150 |
Page 151 |
Page 152
