NATURAL INGREDIENTS 55
Variations of skin hydration after 30 days **
25 20 15 10 5 0
+18.8% p< 0.01
Deep hydration test reults 45.9%
-0.5%
Pyrus malus Juice 5%
Placebo
Figure 5: Percentage variations of skin hydration after 30 days of repeated application of Pyrus malus juice 5% compared to placebo. Skin hydration was measured at baseline and after 30 days
induced a dose-dependent increase in cell metabolic activity compared to both untreated and glycerin-treated controls. ■ At 0.01%, viability increased to ˜105.6% vs.
˜102.9% for glycerin (p = 0.0198) ■ At 0.05%, viability reached ˜106.8% vs. ˜103.7% (p = 0.0029)
■ At 0.1%, although an upward trend was
observed (˜107.5%), the difference from glycerin (˜104.6%) was not statistically significant (p = 0.126) ■ At 0.5% and 1%, viability increased further
(˜107.9% and˜109.8%, respectively), with both comparisons showing statistical significance
compared to glycerin (p = 0.0344 and p = 0.0374, respectively) These results confirm that Pyrus malus juice
significantly enhances mitochondrial activity, indicating improved cellular vitality and potential regenerative support. Mitochondrial function was assessed by measuring metabolic activity, which showed a significant increase in human keratinocytes treated with Pyrus malus juice. This increase suggests enhanced cellular viability and reflects the revitalising potential of the treatment. Such effects are particularly meaningful in the field of skin care, where boosting keratinocyte
50 40 30 20 10 0
43.2% * T0 ■ T30 days ■ ** 44.1% * 42.3%
Pyrus malus Juice 5%
Placebo
Figure 6: Bar chart comparing skin hydration levels at baseline (T0) and after 30 minutes (T30 mins) following application of Pyrus malus juice 5% versus placebo
vitality is linked to improved barrier function, accelerated wound healing, and greater resistance to oxidative stress. Notably, the study was conducted in serum-free
conditions, emphasising the intrinsic bioactivity of the test substance without interference from external growth factors. The use of glycerin as a comparator enabled us to distinguish the specific contribution of the apple-derived bioactives from the solvent vehicle, strengthening the conclusion that Pyrus malus juice displays biological activity. The dose-response relationship observed
further supports the hypothesis of receptor- mediated or concentration-dependent uptake mechanisms, potentially involving phytohormones or secondary metabolites known to interact with mammalian cells.
In vivo hydration measurements7,8,9,10,11,12,13 3.2.1 Superficial hydration - T1 (30 min post- application) ■ Active Product: Increased from 43.7 CU to 50.6 CU (+15.8%), p < 0.05 ■ Placebo: Decreased from 43.2 CU to 39.7 CU (-8.1%), p < 0.05 ■ Comparison: Significant difference between active and placebo groups (p = 0.001)
Variation of deep hydration registered after 30 minutes from the single application
4 3 2 1
0 -1 -2 -3
Pyrus Malus Juice 5% Placebo
Figure 7: Bar chart illustrating the percentage change in skin hydration 30 minutes after a single application of Pyrus malus juice 5% compared to placebo
www.personalcaremagazine.com ** +1.8% A statistically significant increase
(improvement) in the basal values of skin hydration was evidenced after 30 minutes from the single application of the Pyrus malus juice 5% product. A statistically significant decrease (worsening) was registered in the area treated with the placebo.
T2 (after 30 days of repeated application) ■ Active Product: Increased from 43.7 CU to 51.9 CU (+18.8%), p < 0.05. ■ Placebo: Slight decrease from 43.2 CU to 43.0 CU (-0.5%), p > 0.05. ■ Comparison: Statistically significant difference (p < 0.01). This suggests a rapid and sustained hydration effect by the active formulation.
Deep hydration after single application (30 minutes) ■ Active Product: Increased from 43.2% to 45.0% (+1.8%), p < 0.05 ■ Placebo: Decreased from 44.1% to 42.3% (-1.8%), p < 0.05 ■ Comparison: Statistically significant (p < 0.01) The statistical comparison between the
variations recorded at 30 minutes from the single application evidenced a statistically significant difference between the product Pyrus malus juice 5% and the placebo.
Results after 30 Days of repeated application ■ Active Product: Increased from 43.2% to 50.9% (+7.7%), p < 0.01 ■ Placebo: Increased modestly from 44.1% to 46.9% (+2.8%), p > 0.05 ■ Comparison: Statistically significant (p = 0.01) A statistically significant increase
(improvement) in the basal values of skin deep hydration was evidenced after 30 days of repeated application of the Pyrus malus juice 5% product. No significant variation was registered in the area treated with the placebo.
Discussion The methodology employed in this study offers robust evidence of the bioactivity of fruit-derived cosmetic ingredients. At the cellular level, Pyrus
April 2026 PERSONAL CARE MAGAZINE
Variation in hydration (%)
Percentage Change in Skin Hydration
Hydration (%)
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