56 NATURAL INGREDIENTS
Hydration changes after 30 days *
** 50.9% 50 43.2% 40 30 20 10 0 Pyrus Malus Juice 5% Placebo
Figure 8: Bar chart showing mean skin hydration values at baseline (T0) and after 30 days (T30days) of treatment with Pyrus malus juice 5% versus Placebo
malus juice extract induced a significant increase in HaCaT cell viability and mitochondrial activity. This suggests that the compound not only supports cell survival but may also stimulate proliferation and tissue regeneration. Given that keratinocytes form the majority of the epidermis, their revitalization directly translates to improved skin texture and function. The in vivo findings corroborate these mechanistic insights. Skin hydration, a critical marker of barrier function and cosmetic appeal, improved significantly with use of the active formulation. Both surface and deep hydration metrics confirmed that the formulation provides immediate and lasting effects, with clear advantages over the placebo. Of particular interest is the alignment between
in vitro and in vivo outcomes. The mitochondrial stimulation observed in HaCaT cells reflects enhanced biological vitality, which in the clinical setting manifests as improved skin hydration and potentially improved barrier repair. Furthermore, these results are consistent with
literature supporting the dermatological benefits of fruit extracts. Apple polyphenols have been shown to exhibit antioxidative, anti-inflammatory, and barrier-strengthening properties. Their inclusion in topical formulations aligns well with consumer demands for clean, effective skincare products backed by science.
Conclusion The present study offers robust experimental evidence supporting the efficacy of fruit-derived cosmetic ingredients, with particular emphasis on Pyrus malus (apple) juice, as multifunctional agents capable of delivering both revitalising and moisturising benefits to the skin. Through a combination of in vitro and in vivo methodologies, it was demonstrated that the increased mitochondrial activity and cellular vitality observed in keratinocyte cultures translate into clinically relevant improvements in cutaneous hydration
PERSONAL CARE MAGAZINE April 2026 44.1% n.s. 46.9% 10 8 6 4 +2.8% 2 0 Pyrus Malus Juice 5% Placebo
parameters. These findings not only validate the functional bioactivity of such natural ingredients, but also underscore their potential as effective components in the formulation of advanced cosmetic products. The outcomes of this investigation contribute
Figure 9: Deep hydration variation after 30 days of repeated application 2019;41(3):300310
3. Vandorou M et al. A review on apple pomace bioactives for natural functional food and cosmetic products with therapeutic health- promoting properties. International Journal of Molecular Sciences. 2024; 25(19), 10856
meaningfully to the expanding corpus of scientific knowledge that advocates for the incorporation of plant-derived, biologically active compounds within the field of dermatology and cosmetology. The use of fruit-based actives, such as Pyrus
malus juice, represents a convergence between contemporary consumer preferences—particularly the increasing demand for ‘clean’, sustainable, and naturally sourced skincare solutions—and the rigorous scientific evaluation of product efficacy and safety. This alignment is critical for the future development of cosmetic formulations that are not only environmentally and ethically responsible but also supported by empirical data. Looking forward, future research efforts should
aim to explore additional dimensions of these ingredients’ effects, including their long-term impact on skin barrier homeostasis, their influence on biochemical and structural markers of cutaneous ageing, and their capacity to act synergistically when combined with other well-characterized bioactive molecules. Such investigations will be instrumental in
optimizing formulation strategies and in advancing the scientific understanding of how naturally derived compounds can be harnessed to enhance skin health and appearance in a safe, effective, and sustainable manner.
4. Brglez Mojzer E et al. Polyphenols: Extraction methods, antioxidative action, bioavailability and anticarcinogenic effects. Molecules. 2016;21(7):901
5. Interdonato L et al. Targeting Nrf2 and NF-κB Signaling Pathways in Inflammatory Pain: The Role of Polyphenols from Thinned Apples. Molecules. 2023; 28, 5376
6. Report CH-0810-2022 – ChemService. Revitalizing Effect of Hormo Fruit: In Vitro Study with HaCaT Cells on Hormo Fruit Apple and Strawberry
7. Report 203/22/01-02 rev1 - Instrumental Evaluation of the Moisturizing Efficacy after Single and Repeated Application (Active vs Placebo)
8. Berardesca E, Loden M, Serup J, Masson P, Rodrigues LM. The revised EEMCO guidance for the in vivo measurement of water in the skin. Skin Res Technol. 2018;24(3):351–358
9. COLIPA (European Cosmetics Association). Guidelines for the evaluation of the efficacy of cosmetic products. Brussels: COLIPA; May 2008
10. Seidenari S. Diagnostica non invasiva in dermatologia. Milano: EDRA; 1998
PCM
References 1. Johnson W Jr et al. Safety Assessment of Apple- Derived Ingredients as Used in Cosmetics. J Cosmet Sci. 2023; 74(1 Suppl): 36S–56S
2. Nešić I et al. Standardized wild apple fruit extract as a bioactive agent in dermocosmetic products for efficacy skin hydration – In vitro and in vivo evaluation. Int J Cosmet Sci.
11. Serup J, Jemec GBE. Handbook of noninvasive methods and the skin. Boca Raton, FL: CRC Press; 1995
12. August S, Granier S, Tighe MP, Tbaily LW, Chowdhury S, Ahlbom H. A clinical investigation of the performance and safety of Epaderm®, an emollient cream. Clin Cosmet Investig Dermatol. 2021;14:909–920
13. Mayrovitz HN, Wong J, Fasen M. Age and hydration dependence of jowl and forearm skin firmness in young and mature women. J Cosmet Dermatol. 2017;17(6):1262–1270
www.personalcaremagazine.com +7.7% * T0 ■ T30 days ■
Variations of deep hydration registered after 30 days of repeated application
Hydration (%)
Variation in Hydration (%)
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