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SKIN PROTECTION 181 Normal conditions UV stress model with presence of Sunburn Cells


damaging effects of oxidative stress by reducing the level of sunburn cells, keratinocytes that have been damaged by UV radiation, by 62% after UV exposure, performing more effectively than the positive control, vitamin E. It also reduced levels of 8- OHdG, a marker of oxidative stress, and CPD, a major form of DNA damage, by 30% and 80%, respectively, after UV exposure. The results show that this Green Tea biomass is able to promote skin barrier strengthening and superior antioxidation properties.


50 ug/ml reduced the effects of UV stres


Figure 5: Morphology of normal RHE, RHE exposed to UV stress, and after pre-treatment with green tea.


not present, however, after exposure to UV, sunburn cells are formed. When the RHE is pre-treated with Green Tea and then exposed to UV, there is a reduced level of sunburn cells present (Fig 5). 50 µg/ml of Green Tea reduced the level of sunburn cells by 62% in a UV stress model, performing more effectively than the positive control, vitamin E (Fig 6). Under normal conditions there is a small level of 8-OHdG present however, after exposure to UV, the level of 8-OHdG increases. When the RHE is pre-treated with Green Tea and then exposed to UV, there is a reduced level of 8-OHdG. 50 µg/ml of Green Tea reduced the level of 8-OHdG by 30% in a UV stress model.


Continuing with the UV stress study, after


exposure to UV, the level of CPD increases. When the RHE is pre-treated with Green Tea and then exposed to UV, there is a reduced


80 70 60 50 40 30 20 10 0


Figure 6: Evaluation of sunburn cells. April 2020


level of CPD. 50 µg/ml of Green Tea reduced the level of CPD by 80% in a UV stress model, performing as effectively as the positive control, vitamin E.


Discussion


Caspase-14 and Filaggrin are both involved in skin barrier health and UV protection. More specifically, Caspase-14 is an enzyme involved in the reinforcement of the skin barrier and protection against UVB photodamage6


protein involved in hydration and decreasing UV sensitivity7


, while Filaggrin is a structural . The Green Tea active is able


to restore normal skin barrier function by reversing the down regulation of Caspase-14 and Filaggrin in the atopic dermatitis model as effectively as the positive control (Tofacitinib), while normalising TEWL and pH. Green Tea is also able to reverse the


n Normal n UV Stress n 50 µg/ml Vitamin E (Positive Control) n 50 µg/ml


Conclusion Green Tea extracted via SWE produces a highly effective botanical active that is responsibly sourced and can provide a new twist on traditional green tea actives. The subcritical water extraction used yields a rich bioactive composition resulting in high biological efficacy. Green Tea supports skin barrier health with the restoration of key skin barrier building blocks, Caspase-14 and filaggrin, which are important for barrier reinforcement and UV protection. With a healthy and intact skin barrier, skin is able to fight external aggressors and prevent the signs of oxidative stress. It also reduces Sunburn Cells more effectively than vitamin E after UV exposure. This Green Tea extract is a powerful active that can support antioxidation and protection claims and can be marketed as a fresh new take on conventional green tea that outperforms the gold standard, vitamin E.


PC


References 1 Baek J, Lee MG. Oxidative stress and


antioxidant strategies in dermatology. Redox Rep. 2016; 21(4):164-9


2 Parrado C, Mercado-Saenz S, Perez-Davo A, Gilaberte Y, Gonzalez S, Juarranz A. Environmental Stressors on Skin Aging. Mechanistic Insights 2019; 10:759.


3 Burke KE. Mechanisms of aging and development-A new understanding of environmental damage to the skin and prevention with topical antioxidants. Mech Ageing Dev. 2018; 172:123-130.


-62%


4 McDaniel D, Farris P, Valacchi G. Atmospheric skin aging-Contributors and inhibitors. J Cosmet Dermatol. 2018; 17(2):124-137.


5 Gong Y, Zhang X, He L, Yan Q, Yuan F, Gao Y. Optimization of Subcritical Water Extraction Parameters of Antioxidant Polyphenols From Sea Buckthorn (Hippophaë rhamnoides L.) Seed Residue. J Food Sci Technol. 2015; 52(3): 1534-1542.


6 Denecker G, Hoste E, Gilbert B, et al. Caspase- 14 protects against epidermal UVB photodamage and water loss. Nat Cell Biol. 2007; 9(6):666-74.


7 Mildner M, Jin J, Eckhart L, et al. Knockdown of filaggrin impairs diffusion barrier function and increases UV sensitivity in a human skin model. J Invest Dermatol. 2010; 130(9):2286-94.


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Sunburn Cells (UA)


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