SUN CARE
production in M. furfur, but if in addition to sun radiation, the M. furfur culture is treated with 20% of the ingredient, the increase is 18 times higher (Figure 3). In a flow cytometry assay, cultures of S.
epidermidis and M. luteus exposed to sun radiation produced more ROS than their non-irradiated controls. But under the same exposure in the presence of 20% of the active, a reduction of ROS production was observed, by 67% in S. epidermidis, and by 19% in M. luteus, compared to the irradiated controls which were not treated with the ingredient. Finally, we analysed the effect of the
microbial photo-secretome (PS) on ROS and IL-6 production on keratinocytes, in different condition: effect of the treatment of keratinocytes with 1% of each PS, effect of irradiating the keratinocytes at the same time they were treated with 1% of each PS, and effect of the active on keratinocytes irradiated and at the same time treated with 1% of each PS (Figure 4).
Clinical evaluation An in vivo test performed with 20 volunteers with signs of photoageing, summer tan and ages between 49 and 67. Double-blind and placebo-controlled assay, hemi-facial application, 1% PhotoBiome dosage, with two daily applications during 28 and 56 days. The test was carried out in Italy in the end
of the summer season so the sun exposure and the damage on the volunteers’ skin were maximized. The variation in the Individual Tipology
Angle (ITA) was measured in order to study the skin pigmentation during the treatment, both in the face and in the dark hyperpigmented spots (CM-700D colorimeter from Konica Minolta). Furthermore, the skin firmness and elasticity
were assessed by cutometry, and by skin profilometry and PRIMOS 3D analysis, the variation in wrinkle depth in the crow’s feet and in the nasolabial regions (eye contour and bar code areas) were also analysed (Figures 5 and 6).
Conclusion The active ingredient Photobiome protects the cutaneous microbiota involved in fighting the sings of photoageing, at the same time it stimulates the antioxidant and photo- protecting microbial metabolism under sun exposure. This allows a new approach in skin care and in the prevention of the photoageing, where taking care of the skin microbiome we can recover a more smooth, luminous, firm and elastic skin even during and after the sun exposure.
With this 100% natural active from
pomegranate and extremophile cotton stem cells, we obtain a well-ageing effect even under sun radiation exposure, through the positive modulation of the cutaneous microbiota involved in reinforcing the natural skin defence under sun exposure: wrinkles reduction, skin firmness and elasticity increase, and reduction of post-sun exposure hyperpigmentation on skin and on dark spots.
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References 1. Li Z et al. New Insights Into the Skin Microbial Communities and Skin Aging. Front. Microbiol. 2020, 11, 565549
2. Howard B et al. Aging-Associated Changes in the Adult Human Skin Microbiome and the Host Factors that Affect Skin Microbiome Composition. J. Invest. Dermatol. 2022, 142: 1934-1946
3. Luna PC. Skin Microbiome as Years Go By. Am. J. Clin. Dermatol. 2020, 21 (Suppl. 1): 12-17
4. Yang Y et al. Advances in the human skin microbiota and its roles in cutaneous diseases. Microb. Cell Factories. 2022, 21, 176
5. Taner K et al. Bioprospecting the Solar Panel Microbiome: High-Throughput Screening for Antioxidant Bacteria in a Caenorhabditis elegans Model. Front. Microbiol., Sec. Extreme Microbiology. 2019, 10:986
6. Negari I et al. Probiotic activity of Staphylococcus epidermidis induces collagen type I production through FFaR2/p-ERK signaling. Int. J. Mol. Sci. 2021, 22(3):1414
7. Mohana D et al. Antioxidant, antibacterial, and ultraviolet-protective properties of carotenoids isolated from Micrococcus spp. Radiat. Protect. Environ. 2013, 36(4):168-174
8. Greenblatt C et al. Micrococcus luteus - Survival in amber. Microb Ecol. 2004, 48:120-7
9. Hug D et al. The degradation of L-histidine and trans- and cis-urocanic acid by bacteria from skin and the role of bacterial cis- urocanic acid isomerase. J. Photochem. Photobiol. B. 1999, 8:66-73
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10. Patra V et al. Potential of Skin Microbiome, Pro- and/or Pre-Biotics to Affect Local Cutaneous Responses to UV Exposure. Nutrients. 2020, 17-12(6):1795
11. Kim D et al. Combination of Bifidobacterium longum and galacto- oligosaccharide protects the skin from photoaging. J. Med. Food. 2021, 24(6): 606-616
12. Gaya P et al. Bifidobacterium pseudocatenulatum INIA P815: The first bacterium able to produce urolithins A and B from ellagic acid. Journal of Functional Foods. 2018, 45:95-99
13. Vini R et al. Urolithins: The Colon Microbiota Metabolites as Endocrine Modulators: Prospects and Perspectives. Front Nutr. 2022, 2;8:800990
14. Chun-Feng L et al. Antiaging Effects of Urolithin A on Replicative Senescent Human Skin Fibroblasts. Rejuvenation Res. 2019, 22(3):191-200
15. Chong Z et al. Identification of polyphenols that repair the ultraviolet-B-induced DNA damage via SIRT1-dependent XPC/XPA activation. J. Funct. Foods. 2019, 54:119-127
16. Wenjie L et al. Urolithin A protects human dermal fibroblasts from UVA-induced photoaging through NRF2 activation and mitophagy. J. Photochem. Photobiol. B. 2022, Biology Volume 232:112462
17. Youngchim S et al. The role of L-DOPA on melanization and mycelial production in Malassezia furfur. PLoS One. 2013, 7;8(6):63764
18. Mayser P et al. Decreased susceptibility of Malassezia furfur to UV light by synthesis of tryptophane derivatives. Antonie Van Leeuwenhoek. 1998, 73(4):315-9
19. Gaitanis G et al. Novel Application of the Masson-Fontana Stain for Demonstrating Malassezia Species Melanin-Like Pigment Production In Vitro and in Clinical Specimens. J. Clin. Microbiol. 2005, 43(8): 4147- 4151
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