COVER STORY: ASHLAND ADVERTISEMENT FEATURE
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Figure 1: PELNs (in green) in proprietary rosa centifolia extract (TEM)
PSR™ proprietary technology emerges in the field of flower- released exosomes Throughout this exploratory journey, one of our intentions was to determine which of our proprietary technologies was best suited to capturing exosome-like vesicles. Inspired by the science of epigenetic power
of plants, Ashland Plant Small RNA™ technology (PSR™) has demonstrated exceptional abilities in extracting and preserving intracellular plant components. At this stage of investigation, it seems that this technology also stands out for its ability to enrich botanical extracts in PELNs, while preserving their structural integrity and their biological activity.
Proven booster effect of PSR™ rose extract on skin cell derived exosomes The skin is the organ that serves as the outer protective layer and ensures the body’s homeostasis; it can be damaged by external or internal factors, causing a need to subsequently repair and regenerate the skin.5 Scientific evidence is mounting to show
that exosomes play a key role in skin cell communication to activate skin regeneration. Mammalian-derived exosomes have similar structures than PELNs. They differ by their surface marker proteins: CD63, CD81 and CD9
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Control CD9 Control 0.1% Rosaliss
Figure 4: CD9 level in CD63+ exosomes enriching culture medium
www.personalcaremagazine.com
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Figure 2: Particle size distribution and concentration in proprietary rosa centifolia extract (NTA). tetraspanins.1
They are increasingly being
described for their therapeutic potential in the field of regenerative medicine.6 With this in mind, we studied a PSR™ rose
extract, rosaliss™ biofunctional, to help the skin increase its self-repair capacity, to determine whether it could enhance exosome signaling in keratinocytes. In vitro studies were performed on
keratinocytes cultures. After two days of extract application, exosomes were isolated, immunoprecipitated with CD63+ beads and finally detected by western blot with the CD9 marker. Results showed a significant increase of exosome release as shown below, NTA indicated nanoparticle size peak at 94.1 nm.
Tracing the path of flower-released exosomes in skin care These initial findings encourage solvers from Ashland biofunctional research to continue assembling bundles of clues that will lead to a definitive conclusion regarding the presence and bioactivity of PELNs in our flower extracts. As research continues, we can rely on the
strength of our local, sustainable sourcing strategy. Our solid and reliable partnerships with flower producers in Provence and the Grasse region give us access to exceptional flowers, and we are well on our way to unlocking the full biological power of their PELNs for the beauty and health of the skin.
+28*
References 1. Bai C, Liu J, Zhang X, Li Y, Qin Q, Song H, Yuan C, Huang Z. Research status and challenges of plant-derived exosome- like nanoparticles. Biomedicine & Pharmacotherapy. 2024 174, 116543
0.1% Rosaliss
2. Liu Y, Ren C, Zhan R, Cao Y, Ren Y, Zou L, Zhou C, Peng L. Exploring the Potential of Plant-Derived Exosome-like Nanovesicle as Functional Food Components for Human Health: A Review. Foods. 2024, 13, 712
3. Kim J, Li S, Zhang S, Wang J. Plant-derived exosome-like nanoparticles and their therapeutic activities. Asian J Pharm Sci. 2022 Jan;17(1):53-69
4. Sarasati A, Syahruddin MH, Nuryanti A, Ana ID, Barlian A, Wijaya CH, Ratnadewi D, Wungu TDK, Takemori H. Plant-Derived Exosome-like Nanoparticles for Biomedical
Ashland 500 Hercules Road, Wilmington, DE 19808, USA
www.ashland.com
October 2025 PERSONAL CARE
Figure 3: PSR™ technology applied on rosa centifolia
Applications and Regenerative Therapy. Biomedicines. 2023 Mar 29;11(4):1053
PC
5. Tienda-Vázquez MA, Hanel JM, Márquez- Arteaga EM, Salgado-Álvarez AP, Scheckhuber CQ, Alanis-Gómez JR, Espinoza-Silva JI, Ramos-Kuri M, Hernández-Rosas F, Melchor- Martínez EM, Parra-Saldívar R. Exosomes: A Promising Strategy for Repair, Regeneration and Treatment of Skin Disorders. Cells. 2023 Jun 14;12(12):1625
6. Sreeraj H, AnuKiruthika R, Tamilselvi KS, Subha D. Exosomes for skin treatment: Therapeutic and cosmetic applications. Nano TransMed. 2024; Vol 3
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