ANTI-POLLUTION Bleached with EDDS shampoo ■ Bleached without EDDS shampoo ■
1.80 1.60 1.40 1.20 1.00
0.80 0.60 0.40 0.20 0.00
5 10 20 Hair washes
Figure 8: The amount of copper removed from bleached hair after consecutive washes determined using AAS
moiety in pheomelanin. Pigment Cell & Melanoma Research. 2012; 25: 434-445
9. Sebetić K, Sjerobabski Masnec I, Cavka V, Biljan D, Krolo I. UV damage of the hair. Coll Antropol. 2008; Oct;32 Suppl 2:163-5
10. Schram E, Moore S, Bigwood EJ. Chromatographic determination of cystine as cysteic acid. Biochem J. 1954; May;57(1):33-7
11. Hilterhaus-Bong S, Zahn H. Contributions to the chemistry of human hair. 1. Analyses of cystine, cysteine and cystine oxides in
30 40 0.3
0.25 0.2
0.15 0.1
0.05 0
EDDS GLDA Chelant
Figure 9: The average change in copper content over three hair switches after 60 washes with the H0167 test shampoo containing different chelants or a no chelant control
untreated human hair. International Journal of Cosmetic Science. 1987; 9: 101-110
12. Tomas X, Nogueras M, Bartolome A, Ferrando J. Pseudo Green Hair. Int J Trichology. 2022 Mar-Apr;14(2):65-67
13. Marsh JM, Davis MG, Flagler MJ, Sun Y, Chaudhary T, Mamak M, McComb DW, Williams REA, Greis KD, Rubio L, Coderch L. Advanced hair damage model from ultra- violet radiation in the presence of copper. Int J Cosmet Sci. 2015; 37: 532-541
14. Tilley A, McHenry MP, McHenry JA, Solah V, Bayliss K. Enzymatic browning: The role of substrates in polyphenol oxidase mediated browning. Curr Res Food Sci. 2023; Oct 20;7:100623
15. Cavallaro G, Milioto S, Konnova S, Fakhrullina G, Akhatova F, Lazzara G, Fakhrullin R, Lvov Y. Halloysite/Keratin Nanocomposite for Human Hair Photoprotection Coating. ACS Appl Mater Interfaces. 2020; May 27;12(21):24348- 24362
EDTA Control
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October 2025 PERSONAL CARE
Copper removed % m/m
Copper removed % m/m
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