56 ANTI-POLLUTION
statistically significant (p<0.01) smaller peak area than the control, demonstrating that the control hair was more damaged by the UV exposure.
Atomic absorption spectroscopy (AAS) Atomic absorption spectroscopy is widely used in the detection of metals for quality control purposes across many industries. AAS can detect low levels of metal in a sample, therefore this method was well suited for determining the copper content of the hair switches across all stages of testing. Hair samples were soaked in 5% copper
sulphate solution for 24 hours before being washed five, ten, 20, 30, and 60 times with the EDDS containing shampoo. Initially and after each set of washes, a sample was cut off the bottom of the switch before 0.1g of the hair was digested using nitric acid in a microwave digester for 75 minutes at 180°C before being analysed for copper content by AAS. A comparison of the copper content of
bleached and virgin hair showed that bleached hair took up 4 times the amount of copper than virgin hair. Brown virgin hair showed a statistically significant reduction of copper content after ten washes (p<0.05) from the unwashed hair and did not show a further decrease for the remainder of the washes. Bleached hair was found to lose copper
as easily as it was taken up, as there was a statistically significant reduction in copper content after five washes (p<0.05) and ten washes (p<0.01) from the unwashed hair, a trend which continued up to and including 30 and 60 washes. This procedure was then repeated using
virgin brown hair which was washed 60 times with a shampoo containing EDDS, GLDA, EDTA or a control without chelant. EDDS produced a significant (p<0.05) reduction in copper content
The degradation of this hydrogen peroxide
would have generated the highly reactive hydroxyl radical which itself causes oxidative damage within the hair fibre. The reduced production of hydroxyl radicals therefore leads to less oxidative damage throughout the hair fibre, resulting in better hair health.
References 1. Li Q. Effect of forest bathing trips on human immune function. Environ Health Prev Med. 2010; Jan;15(1):9-17
2. Chandra P, Wolfenden LL, Ziegler TR, Tian J, Luo M, Stecenko AA, Chen TC, Holick MF, Tangpricha V. Treatment of vitamin D deficiency with UV light in patients with malabsorption syndromes: a case series. Photodermatol Photoimmunol Photomed. 2007 Oct;23(5):179-85
in comparison to the control, whereas the other biodegradable chelant GLDA did not produce a significant reduction. EDTA produced a significant (p<0.05)
reduction in copper content as would be expected as it has been used in medical treatments for chlorotrichosis, however EDDS can achieve the same effect with superior biodegradability and safety credentials.
Conclusion An array of testing methods has shown that washing the hair with H0167 The Copper Detox shampoo containing Natrlquest E30 (EDDS) can prevent damage to the hair fibre from UV radiation. Natrlquest E30 does this by reducing the copper content in the hair fibre, removing the ability of copper to participate in the degradation of hydrogen peroxide which is formed in the hair during the UV exposure of amino acids.
93.5 93.0 92.5 92.0 91.5 91.0 90.5 90.0 89.5 89.0
1090 1080 1070
1060 Wavenumber (cm-1)
1050 1040 1030 1020 1010
Figure 7: FTIR spectra of bleached and virgin hair alongside brown hair which has been washed with or without an EDDS containing shampoo and exposed to UV light for 72 hours
PERSONAL CARE October 2025
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3. Veleva BI, van Bezooijen RL, Chel VGM, Numans ME, Caljouw MAA. Effect of ultraviolet light on mood, depressive disorders and well-being. Photodermatol Photoimmunol Photomed. 2018; 34: 288–297
4. Narayanan DL, Saladi RN, Fox JL. Review: Ultraviolet radiation and skin cancer. International Journal of Dermatology. 2010; 49: 978-986
5. Ruetsch SB, Kamath Y, Weigmann H-D. Chapter 9 - Photodegradation of human hair: a microscopy study, Editor(s): Paolo U. Giacomoni. Comprehensive Series in Photosciences. Elsevier. 2001, Volume 3; Pages 175-205
6. D’Orazio J, Jarrett S, Amaro-Ortiz A, Scott T. UV radiation and the skin. Int J Mol Sci. 2013; Jun 7;14(6):12222-48
7. Lee WS. Photoaggravation of hair aging. Int J Trichology. 2009 Jul;1(2):94-9
8. Wakamatsu K, Nakanishi Y, Miyazaki N, Kolbe L, Ito S. UVA-induced oxidative degradation of melanins: fission of indole moiety in eumelanin and conversion to benzothiazole
■ EDDS washed and 72h UV exposed ■ EDDS washed and 72h UV exposed ■ Bleached hair ■ Virgin hair
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