(HILIC) A variant of normal-phase chromatography that uses hydrophilic (polar) stationary phase and hydrophobic (mostly organic, typically aprotic) and water miscible mobile phase. Retention increases with hydrophilicity of analytes and the order of elution is opposite to that obtained with reversed-phase chromatography.
(HIC) A form of chromatography whereby a matrix containing hydrophobic groups binds proteins from aqueous solutions to different extents depending on the protein structures and a range of controllable factors including concentrations of salts, pH, temperature and organic solvents.
197. hyperlipidemia (lipemia) Abnormally high levels of lipids and/or lipoproteins in blood, often associated with genetic factors and diabetes. Reliable bioanalysis of hyperlipidemic samples may be compromised due to the lipid content and method performance is typically checked using several lots of spiked blank hyperlipidemic matrix.
