The Bioanalyis Glossary


366. PrD-LC–MS A bioanalytical method where matrix containing the analyte protein is directly digested using trypsin or another specific protease followed by quantitative analysis of the surrogate peptide by LC–MS/MS; ‘reagent-free’ methods use no immuno- or affinity enrichment of either protein analyte or surrogate peptide. In specific cases, mass spectrometry methods other than MS/MS may be applied.


367. PrD-LC–MS-IC A bioanalytical method where the analyte protein is first purified from the source matrix by an immunoaffinity method followed by proteolytic digestion using trypsin or another specific protease, then by LC–MS/MS analysis of surrogate peptide. This method uses no immuno- or affinity enrichment of the surrogate peptide. In specific cases, mass spectrometry methods other than MS/MS may be applied.


368. PrD-LC–MS-SC A bioanalytical method where matrix containing the analyte protein is directly digested using trypsin or another specific protease followed by immunopurification of the surrogate peptide in the digest, then by quantitative analysis of the purified surrogate peptide by LC–MS/MS.


In specific cases, mass methods other than MS/MS may be applied. 116 Bioanalysis (2020) 12(21) Suppl. 1 future science group spectrometry


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