Definitions 480. ultra-high-performance liquid chromatography


(UHPLC) Makes use of support particles that often have diameters less than 2 µm in size. These smaller particles make it possible to obtain more efficient separations but also increase the pressure that is required to apply the mobile phase through a given length of column.


481. undersampling This describes the transfer of portions of fluid exiting the first dimension column to the second separation column at a rate that is too slow to maintain the integrity of the first dimension separation prior to the sampling process. That is, when these transfers are made too infrequently, we say that the first separation is undersampled, which results in remixing of sample constituents that had already been resolved by the first separation column.


482. unknown A biological sample that is the subject of the analysis with the goal to determine analyte(s) concentrations.


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