190. hook effect The phenomenon typically observed in sandwich ELISAs, whereby particularly high analyte concentrations result in decreased signals compared with slower nominal concentration on the curve, resulting in a hook-shaped curve at the high concentration region.
191. hit Identified molecule that has the ability to bind to its target to bring about a biological response, which may require further development.
192. hit confirmation Series of methods/assays applied to an identified hit molecule to confirm its structure and activity profile.
193. hit identification Process to analyze molecules to discover which has activity against a target.
194. hit-to-lead/lead generation Stage in early drug discovery where small-molecule hits are evaluated and undergo limited optimization to identify promising lead compounds.
