69. blood:plasma distribution ratio The extent to which a drug is distributed between plasma and the red blood cell fractions of blood, often expressed in the blood-to- plasma concentration ratio of the drug.
70. bottom-up proteomics The bottom-up approach implies that the information of a biological sample is reconstructed from individually identified fragment peptides. In this approach, the samples are subjected to proteolytic digestion, typically using trypsin, and protein identification is done by peptide sequencing with MS/MS.
71. bridging immunoassay An immunoassay method for detection of antidrug antibodies (ADAs) that is enabled by the formation of a ternary complex or ‘bridge’ where one arm of the ADA binds to the capture reagent (e.g., solid phase adsorbed or biotin-labeled drug) and the second arm binds to the detection reagent (e.g., hapten-, enzyme- or ruthenium-labeled drug). Often the detection reagent is a labeled version of the capture reagent. An analogous assay may also be used for quantitation of analytes.
