411. response function A mathematical function that adequately describes the relationship between instrument response (e.g., peak area or height ratio) and the concentration (amount) of analyte in the sample. Response function is defined within a given range.
412. retention time (RT) The time taken for an analyte to reach the detector after injection and retention through a chromatographic column.
413. reversed-phase chromatography Type of chromatography employing a nonpolar stationary phase and a polar mobile phase. Hydrophilic analytes have limited affinity for the stationary phase and are eluted first, while lipophilic analytes tend to adsorb to the stationary phase and are eluted by decreasing the polarity of the mobile phase.
