Definitions


487. van Deemter equation H = A + B/u + Cu; the A term represents the contribution from eddy diffusion. Eddy diffusion results from radial flow inequalities through a packed bed. The B term (B/u) represents the contribution from longitudinal diffusion. The C term (Cu)


represents the


contributions from resistance to mass transfer in the stationary and mobile phases. u is mobile phase velocity (mm/s). An optimum mobile phase velocity exists for a column at which its highest efficiency would be realized. While the A term is somewhat fixed for each column, the B and C terms play a significant role in column efficiency.


488. very-high-pressure liquid chromatography (VHPLC) See ‘UHPLC’.


489. volumetric absorptive microsampling Collection of a fixed volume of blood using an absorbent tip.


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