76 HAIR CARE
20 15 10 5 0
Vehicle Control ■ 0.5% Oat Peptide Powder ■ +19%***
Control ■ 1% Oat Peptide Powder ■ 1.5 -26%* +11%** 1.0 +4%** +1%NS 0.0 Collagen I Collagen III Collagen V Elastin
Figure 2: Effect of OPP on structural biomarkers of the dermis. ANOVA test, Significant: ***=p<0.001, **=p<0.01 (99%), NS= non-significant
3,000 Da) range, with the maximum molecular weight of 5,000 Da. Medium molecular weight peptides aid in the repair and maintenance of the scalp’s protective barrier.3 Low molecular weight peptides penetrate
deeply into the scalp, promoting cellular repair and regeneration.3
OPP peptide distribution
has been proven to enhance scalp health by facilitating both surface-level barrier reinforcement and deep tissue regenerative processes.
OPP is an eco-friendly product, with
raw material oat by-product sourced from sustainable farms in Europe. It is produced using a novel, biotechnology process with a focus on minimising waste generation. The powder form not only makes it convenient to use but also eliminates the need for preservatives. OPP is readily biodegradable reducing its environmental impact. This work presents its efficacy to revitalise the
hair from the scalp by strengthening scalp health, exhibiting reduced hair fall activity resulting in fuller-looking hair with minimised shedding.
Investigating scalp revitalisation through collagen and elastin synthesis with OPP Collagen is a protein that is a fundamental building block in the human body, playing a crucial role in the health of the skin (scalp). Regarding scalp health, collagen strengthens hair follicles, prevents hair thinning and hair fall, enhances blood circulation and moisturises the scalp.4 Elastin is another important protein found in
the skin. Its primary function is to provide elasticity to tissues and plays a crucial role in maintaining health and integrity of tissues, to support blood vessel function and elasticity of the scalp.5 An ex vivo study was performed to evaluate
the effect of OPP on collagen I, III and V and elastin synthesis. Skin explants were obtained from a 37 year-old female Caucasian. The explants were divided into 3 experimental groups (Control: Untreated, Vehicle control: Distilled water, 0.5% OPP in vehicle control), with products applied topically twice a day for three days. Sections of the explants were prepared
and fixed, followed by incubation with primary antibodies targeting collagen (Col1, Col3, Col5)
PERSONAL CARE November 2024 SRD5A1 SRD5A2 SRD5A3
Figure 3: Effect on 5a-reductase gene expression after 1 day of OPP treatment in HFDPC. ANOVA test, Significant: *=p<0.05 (95%), NS=non-significant
and elastin (Ela). Secondary antibodies coupled to fluorophores were used for detection and cellular nuclei were labelled with DAPI. Excess antibodies and DAPI were removed and fluorescent images were captured using an epi- fluorescent microscope. The application of 0.5% OPP significantly
increases, in comparison to control: ■ Collagen I level by 4%. ■ Collagen III level by 1%. ■ Collagen V level by 19%. ■ Elastin level by 11%. OPP contributes to enhancing the structural
framework of the scalp and boosts collagen I, III, V and elastin. OPP’s role in enhancing collagen and elastin synthesis underscores its potential to fortify the scalp’s structural integrity, essential for reducing hair fall.
Reducing hair fall activity with OPP Testosterone is converted into dihydrotestosterone (DHT) by the enzyme 5a-reductase. DHT then binds to androgen receptors, causing gradual miniaturisation of hair follicles by reducing their blood flow. With ageing, concentrations of DHT can increase. Elevated DHT levels lead to increased
binding to receptors in hair roots, which restricts oxygen and nutrient supply, shortening the anagen (growth) phase of hair. Over time, the hair bulb will shrink and regress quicker, until the hair eventually falls out.6 An in vitro study assessed the inhibiting
capacities of OPP on the gene expression of steroid 5 alpha-reductase 1 (SRD5A1), 2 (SRD5A2) and 3 (SRD5A3). Human follicle dermal papilla cells (HFDPC), isolated from the scalp of a 56-year-old Caucasian male, were used. 1% OPP diluted in water was used as the test material groups and kept in follicle dermal papilla cell growth medium S5. After one day of application, total RNA was purified, quantified and complementary DNA (cDNA) was synthesised. Relative gene expression of SRD5A1, SRD5A2 and SRD5A3 was assessed by quantitative polymerase chain reaction (qPCR). HFDPC exposed to OPP underwent a
downregulation of steroid 5a-reductase 1 and 3. 1% OPP significantly reduces the expression of SRD5A1 and SRD5A3 by 26% and 27% versus untreated control, respectively. It is widely
accepted that hair loss is related to vascular and hormonal components and that elevated androgen levels seem to be responsible for follicular regression.7 Anti-androgens exhibit inhibitory activity on
5a-reductase and block androgen receptors, which may be useful for the treatment of hair loss.8
OPP exhibits reduced hair fall activity,
substantiated by a significant downregulation of steroid 5a-reductase 1 and steroid 5a-reductase 3. OPP’s ability to downregulate SRD5A1 and SRD5A3 highlights the potential to reduce hair fall and restore hair vitality.
Preventing hair fall with OPP An average person loses between 40 to 80 hairs per day. Hair loss is characterised by losing over 100 hairs a day. There are 2 types of hair fall, gradual (hormone-dependent process) and sudden (nutritional deficiency, poor blood circulation or inflammatory pathway). If left untreated, initially subtle hair loss can become more pronounced, leading to thinner and fewer hairs. A blind study was performed to evaluate the
efficacy of OPP on hair density, compared with a placebo. Two panels of 20 participants (male and female) aged between 25 to 70 years old, with straight (Asian and Caucasian), thin hair subject to hair loss (male participants with Hamilton scores of 3 to 4, which measure the severity of male pattern baldness and female participants with Savin scores of 2 to 4, which assess the extent of female pattern hair loss) were recruited. Both panels consisted of stressed
participants (with a score less than 15 on the World Health Organisation-five well-being index and a score of at least 14 on the Perceived Stress Scale). Over a period of 120 days, 1 panel applied OPP leave on scalp serum (Aqua, Cellulose Gum, Avena sativa (Oat) Peptide, Phenoxyethanol, Ethylhexylglycerin, Citric Acid) on the whole scalp and the other panel applied a placebo leave on scalp serum (Aqua, Cellulose Gum, Phenoxyethanol, Ethylhexylglycerin, Citric Acid) on the whole scalp. The product was applied once a day, in
the evening (application on the scalp only and without rinsing after the application). Measurements were taken on day 0 (before treatment), after 30, 90 and 120 days.
www.personalcaremagazine.com 0.5 -1%NS -27%*
Biomarkers Levels Induction (% vs Control)
Relative Expression
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