32 TRENDING TECHNOLOGIES
Characterization of plant exosomes through NTA Nanoparticle tracking analysis (NTA) was employed to determine the size distribution and concentration of the exosomes, tracking the movement of nanoparticles in a liquid suspension to provide quantitative measurements. The NTA characterization confirmed that the exosomes from both Centella asiatica and Curcuma longa exhibit optimal sizes and high concentrations, validating their efficacy for advanced skincare and haircare products. The analysis of Curcuma longa exosomes
yielded notable results for both active ingredients, Turmeria Zen and Capilia Longa formulations. The Turmeria Zen formulation of Curcuma longa exosomes was quantified at a billion-level concentration per millilitre. These particles had an average diameter of more than 100 nanometers, demonstrating a high concentration of smaller-sized exosomes. Furthermore, the Capilia Longa formulation
of Curcuma longa exosomes also showed a billion-level concentration of exosomes per millilitre, with an average diameter of more than 100 nanometers. This slightly larger size, coupled with a substantial concentration, suggests a potent formulation for hair care applications. Similarly, for Centella asiatica in the Centella
Reversa formulation, a significant exosome concentration of a billion level per millilitre was quantified, exhibiting an average diameter of more than 100 nanometers.
Clinical evaluation Analysis of regenerative efficacy and global hair growth and density increase With the aim to study the regenerative
A A B
Figure 3: Curcuma longa cell culture exosomes. Images of C. longa exosomes taken by CRYO-TEM (transmission electron microscopy). At x40,000 magnification (A,B)
efficacy and hair growth and density increase of Capilia Longa™, a clinical evaluation was conducted with a panel of 40 Caucasian volunteers, male and female, between 18 and 60 years old. They all showed hair loss (ratio or hairs in Anagen and Telogen phases less than 4 (A/T=4 is considered the normal), with different etiologies, grade Hamilton I-IV in male and female and grade Ludwig I-II in female: ■ Male and female androgenetic alopecia ■ Seasonal alopecia ■ Diffuse alopecia ■ Hair loss due to technical processes ■ Fragility ■ Menopause The clinical in vivo study assessed the
regenerative efficacy of a treatment aimed at increasing hair growth and density. The number of hairs present by unit of area was
B
calculated from images of the scalp taken with the TrichoScan® microcamera at T0, T90 and T150. The results were expressed as the percentage increase in hair density compared to the baseline. The average increase of hair density by 15.3 % and 17.3% at 90 & 150 days, respectively. Also, for the eyebrows and eyelash density
evaluation, Curcuma longa secretome increased the eyelash density up to +200% (threefold) after two and four months and increased the eyebrows density up to +50% (1.5-fold) and +100% (twofold) after two and four months (Figure 5).
In vivo & ex vivo - Analysis of skin hydration and regeneration efficacy with Turmeria Zen For the in vivo test, a panel of 20 volunteers (30–50-year-old) was selected, subjected to intense psychosocial stress. The volunteer selection was done through STAI questionnaire (State-Trait Anxiety Inventory for Adults) and Cortisol test in saliva (Salimetrics® Europe Ltd.). It was a single blind placebo-controlled study, with two applications per day in the face (placebo and Turmeria Zen), for 28 days, during the winter season (January-February) in Poland, thus extra challenging the skin for dryness from rigorous cold in such period. The measurements were taken on the Crow’s feet area.
With the aim to evaluate the regenerative C D
activity of the active, an ex vivo test was conducted on punch biopsies (significant physical stress) to analyze how the active demonstrated regenerative properties on punch biopsies (epidermis removal of 8 mm diameter, leaving the inner 3 mm circle with epidermis). After the application of the active on Days
0, 3 and 5, the biopsies were analyzed on Day 8. The Fig. 6 shows that the active ingredient has a higher epidermal regeneration than the growing factor EGF, as well as higher dermal redensifying and restructuring than EGF.
Figure 4: Centella Reversa cell culture exosomes. Images of C. asiatica exosomes taken by transmission electron microscopy (TEM). At x10,000 magnification (A), the overall morphology of the exosomes is visible. At x20,000 magnification (B), more detailed structural features can be observed. At x30,000 magnification (C), finer structural elements are highlighted, and at x60,000 magnification (D), a highly detailed view of the exosome surface and internal structures is provided
PERSONAL CARE November 2024
In vivo – reversing ageing with Centella Reversa Facial complexion analysis In vivo tests measure biomechanical
www.personalcaremagazine.com
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