66 ANTI-AGEING P7 UVAP7 UVAP7+0.3125% BF UVAP7+0.625% BF UVAP7+1.25% BF UVAP7+2.5% BF
25000 20000 15000 10000 5000 0
+54.33% *** *** *** *** -77.38%
* Green fluorescence-8-OHdG
Cell: Natural aging cells (P7), photoaging cells (UVAP7) Figure 9: Fluorescent photos of 8-OHdG
Ferment Lysate at concentrations ranging from 0.078% to 1.25%, the green fluorescence intensity increased significantly compared with UVA-P7. The total fluorescence intensity was significantly increased by 87.35% at 0.78% concentration and 384.93% at 1.25% concentration, indicating that Bifida Ferment Lysate at concentrations of 0.078% to 1.25% can significantly enhance the fluorescence intensity of HES1.
Degree of DNA damage γH2AX is a marker of DNA double-strand breaks,6
degree of DNA oxidative damage, which is increased in senescent cells.7
and 8-OHdG is a biomarker of the As shown
in Figure 7, P7 cells showed very weak fluorescence after immunofluorescence staining by γH2AX, whereas the fluorescence intensity of individual cells in UVA-P7 increased significantly, especially the green fluorescence localized at the nucleus. The total fluorescence intensity of UVA-P7
increased significantly by 569.71% compared with that of P7, which indicated that stronger DNA double-strand breaks occurred in UVA-P7. After synchronized treatment with Bifida Ferment Lysate at concentrations ranging from 0.625% to 5%, the green fluorescence showed a dose-dependent decrease compared to untreated UVA-P7 (Figure 8). At a concentration of 5%, the fluorescence intensity of UVA-P7 was reduced by 96.63%
P7 UVAP7 Figure 10: Fluorescence intensity of 8-OHdG
compared to P7. Therefore, Bifida Ferment Lysate can significantly reduce DNA double- strand breaks in UVA-P7. Then analyzing 8-OHdG, compared
with P7, the green fluorescence intensity of individual cells in UVA-P7 increased, and the total fluorescence intensity after quantification increased significantly by 54.33%, indicating that UVA-P7 has more 8-OHdG, more DNA oxidative damage, and a higher ageing degree. The green fluorescence intensity of
single UVA-P7 gradually decreased after synchronized treatment with Bifida Ferment Lysate at concentrations ranging from 0.3125% to 2.5%. At a concentration of 2.5% , it was significantly reduced by 77.38%, indicating that Bifida Ferment Lysate can dose-dependently reduce the production of 8-OHdG and decrease DNA oxidative damage in UVA-P7.
Effects on organelles functions The results of MitoBright LT probe staining (red fluorescence) are shown in Figures 11 and 12. The darker staining colour represents higher mitochondrial membrane potential and higher mitochondrial activity and function. When cultured for 24 hours, compared with
P7 cells, the red fluorescence of individual cells in UVA-P7 cells became weaker, and the total fluorescence intensity decreased by 62.29%, indicating that the senescence of UVA-P7 increased. However, after the synchronized treatment
UVAP7+0.078% BF 15000 10000 UVAP7+0.3125% BF UVAP7+1.25% BF UVAP7+5% BF 5000 0 -62.29% * * *** **
with a concentration range of 0.078% to 5% of Bifida Ferment Lysate, the intensity of red fluorescence of individual cells in UVA-P7 was significantly after 24 hours of incubation. This indicates that the 0.078% to 5% concentration of the Bifida Ferment Lysate can continuously enhance mitochondrial activity.
Reduction of SASP MMP-3 is a typical SASP component. Its increased expression can promote the degradation of some protein components in the extracellular matrix and accelerate cellular senescence.8 As shown in Figure 13, compared with P7,
the MMP-3 content in UVA-P7 increased, but with no significant differences, consistent with METTL3 results. Synchronized treatment with Bifida Ferment Lysate at concentrations ranging from 0.01% to 3% also dose- dependently reduced the MMP-3 content in UVA-P7, with a significant difference.
Clinical test of anti-wrinkle and firming efficacy Skin ultrasound It can be seen from Figure 14 that, compared with the high intensity echo (Collagen Intensity) before using the product (0 days), it increased by 21.53%, 22.91%, and 23.00% respectively after using the serum for 14 days, 28 days, and 56 days, with significant differences.
+1263.86% * ***
UVA-P7 + BF
* Red florescence-mitochondria
Cell: Natural aging cells (P7), photoaging cells (UVAP7) Figure 11: Fluorescent photos of mitochondria PERSONAL CARE November 2024
UVA-P7 + BF Figure 12: Fluorescence intensity of mitochondria
www.personalcaremagazine.com
Mitochondria Activity RFU
8-OHdG RFU
P7 UVA-P7 0.3125% 0.625% 1.25% 2.5%
P7 UVA-P7 0.078% 0.156% 0.3125% 0.625%
1.25% 2.5% 5%
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