ANTI-AGEING P7 UVAP7 UVAP7+0.3125% BF UVAP7+0.625% BF UVAP7+1.25% BF UVAP7+2.5% BF
4000 3000 2000 1000 0
-75.68% ** * * *** +384.93%
65
*
* Green fluorescence-8-OHdG
Cell: Natural aging cells (P7), photoaging cells (UVAP7) Figure 5: Fluorescent photos of HES1
Test method In this test, samples were randomly applied to the left and right sides of the face, and the test results before and after application were compared. The single blind test is adopted, and the instruments were used to repeat the test for three times. Participants used two pumps (approximately
0.5 g) twice daily, once in the morning and once in the evening, for 56 days. During this period, other skin care products with anti-wrinkle and firming effects were discontinued. The anti-wrinkle and firming efficacy of 3% Bifida Ferment Lysate Serum was examined by Skin Ultrasound, VISIA, and visual observation by dermatologists before (0 days), 14 days, and 28 days after use.
Data analysis Universal and representative pictures were selected for group comparison in both in vitro and clinical tests. In the clinical tests, the SPSS Statistics 27 was used for statistical analysis, and GraphPad Prism 7.04 was used for drawing. Where, * represents P<0.05, ** represents P<0.01, and *** represents P<0.001, with a significant difference.
■ Improvement rate (%) = | (Mean value after use Mean value before use
- 1) *100%|
In the in vitro tests, the senescent cell-related indicators detected by immunofluorescence
P7 UVAP7 / Figure 6: Fluorescence intensity of HES1
staining were analyzed by Image J software for the total fluorescence intensity of at least three fluorescent pictures. The significant difference between the samples compared with the control group was calculated by TTest method according to the formula mentioned above. Where, * represents P<0.05, ** represents P<0.01, and *** represents P<0.001, with a significant difference.
Results Improvement in overall cell viability It can be seen in Figure 2 that compared with P7 at 24, 48 and 72h, the OD value tested by UVA-P7 decreased, and the cell viability decreased to 67.65%, 58.19% and 63.40% respectively, with the largest difference at 48 hours. In contrast, Bifida Ferment Lysate, at
concentrations ranging from 0.01% to 3%, resulted in UVA-P7 activity increasing dose- dependently at 24, 48, and 72h when treated synchronously. The enhancement rates at 3% reached 132.42%, 300.39%, and 319.35%, respectively, which were significantly different. At the concentration of 0.3%, the tested OD value was equivalent to that of P7.
Content of COL I Fibroblasts can synthesize over 90% COL I in the skin. A decrease in cell viability leads to the reduction in COL I synthesis, which may lead to problems such as wrinkles.4
UVAP7+1.25% BF UVAP7+2.5% BF UVAP7+5% BF
2000 1500 1000 500 0
expression in the cells was selected to be tested at 48 hours. Compared with P7, the red fluorescence
intensity of individual cells in the UVA-P7 was significantly decreased, and the total fluorescence intensity decreased by 66.23% (Figure 3 and 4). This indicates that the COL I expression in UVA-P7 was significantly lower than that of in P7, suggesting a higher degree of cellular senescence in UVA-P7. After treatment with Bifida Ferment Lysate
at concentrations ranging from 0.078% to 1.25%, the intensity of red fluorescence in UVA-P7 significantly increased. The total fluorescence intensity significantly increased at 0.078% and maximized at 0.625% to1.25% concentration, with the enhancement rate of 843.93%, indicating that the 0.078% to 1.25% concentration of Bifida Ferment Lysate can significantly promote the expression of COL I in UVA-P7.
Reduction of nucleus damage HES1 Fluorescence Intensity HES1 is a key transcription factor for inhibiting senescence in fibroblasts and is mainly expressed in the nucleus.5
Compared with The COL I +569.71%
P7 (Figures 5 and 6), the green fluorescence intensity in UVA-P7 decreased, and the total fluorescence intensity decreased by 75.68%, indicating that the degree of senescence of UVA-P7 was greater. After the synchronized treatment with Bifida
-96.63%
UVA-P7 + BF
* ** ** ** * Green florescence yH2AX Cell: Natural aging cells (P7), photoaging cells (UVAP7) Figure 7: Fluorescent photos of γH2AX
www.personalcaremagazine.com Figure 8: Fluorescence intensity of γH2AX November 2024 PERSONAL CARE UVA-P7 + BF
ϒH2AX RFU
HES1 RFU
P7 UVA-P7 0.078%
0.156% 0.3125%
0.625% 1.25%
P7 UVA-P7 0.625% 1.25% 2.5% 5%
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92 |
Page 93 |
Page 94 |
Page 95 |
Page 96 |
Page 97 |
Page 98 |
Page 99 |
Page 100
