ANTI-AGEING Compared with UVAP7, * means p<0.05, ** means P<0.01, *** means p<0.001
40000 30000 20000 10000 0
33.07% ** ** ** *** *** ***
60 55 50 45 40
UVA-P7 + BF Cell: Natural ageing cells (P7), photoageing cells (UVAP7) Figure 13: The content of MMP-3
Wrinkle score As can be seen from the Figure 15, the participants’ grades of eye contour scored by dermatologists were reduced by 5.13% and 7.69% after 28 days and 56 days of product serum using, respectively, compared to the pre- use period (0 days), with a significant difference at 56 days. Taking 45-year-olds as the boundary (this population experiences more severe external exposure and ageing), 16 participants older than 45 years old were analyzed. It was found that, compared to the trial period (0 days), after using the serum for 28 days and 56 days, the dermatologist-scored eye contour grades of these participants were decreased by 7.69% and 11.54%, respectively, with significant differences.
VISIA image The VISIA images of two typical participants were selected for presentation (Figure 16).
Conclusion In this study, the anti-ageing efficacy of Bifida Ferment Lysate was verified through both in vitro and clinical tests. The results indicate that Bifida Ferment Lysate can alleviate cellular senescence and demonstrate excellent performance in firming and anti-wrinkle treatments in clinical tests. As such, Biomenta BF shows promise as an innovative ingredient for anti-ageing products.
0 DAYS
2.7 2.6 2.5 2.4 2.3
Figure 14: The high intensity echo by skin ultrasound
-5.13% -7.69%
ns *
0d (n=30 people)
28d 56d Figure 15: Lines around the eyes scored by dermatologists
References 1. Fitsiou E, Pulido T, Campisi J et al. Cellular senescence and the senescence- associated secretory phenotype as drivers of skin photoaging. J. Invest. Dermatol. 2021;141(4S):1119-1126
2. Kumar P, Nagarajan A, Uchil PD. Analysis of cell viability by the MTT assay. Cold Spring Harbor Protocols. 2018; Jun; 2018(6)
3. Donaldson JG. Immunofluorescence staining. Curr. Protoc. Cell Biol. 2015;69:4.3.1-4.3.7
PC 28 DAYS
4. Sadowska-Bartosz I, Bartosz G. Effect of antioxidants on the fibroblast replicative lifespan in vitro.Oxidative Medicine and Cellular Longevity. 2020;13:6423783.
0 DAYS
5. Zou Z, Long X, Zhao Q et al. A single-cell transcriptomic atlas of human skin aging. Dev. Cell. 2021;56(3):383-397
6. Watanabe S, Li YS, Kawasaki Y et al. Workers’ lifestyles and urinary 8-hydroxydeoxyguanosine as an oxidative stress marker. J. UOEH. 2019;41(4):431-436
7. Valdiglesias V, Giunta S, Fenech M et al. γH2AX as a marker of DNA double strand breaks and genomic instability in human population studies. Mutat. Res. 2013;753(1-2):24-40
8. Pittayapruek P, Meephansan J, Prapapan O et al. Role of matrix metalloproteinases in photoaging and photocarcinogenesis. Int. J. Mol. Sci. 2016;17(6):868
28 DAYS >45 years old
3.25 3
2.75 2.5
-7.69% -11.54%
+21.53% ***
+22.91% *** +23.00% ***
67
0d
14d
28d
56d The high intensity area mainly includes collagen and elastin
*** ***
0d
28d (n=16 people)
56d
GDH, Female, 52 years old
www.personalcaremagazine.com Figure 16: Two typical participants by VISIA images
CBF, Female, 57 years old
November 2024 PERSONAL CARE
MMP-3 PG/mL
Lines around the eyes
High intensity echo
Lines around the eyes
P7 UVA-P7
0.01% 0.03% 0.1% 0.3% 1% 3%
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92 |
Page 93 |
Page 94 |
Page 95 |
Page 96 |
Page 97 |
Page 98 |
Page 99 |
Page 100