TRENDING TECHNOLOGIES
the stem cells of Curcuma longa and Centella asiatica, which offer multiple cosmetic applications. These applications range from reversing ageing and regenerating the skin to revitalizing hair growth and density through their plant growth factors. The Curcuma longa cell cultures developed
by Vytrus Biotech include two cosmetic ingredients, Turmeria Zen™ and Capilia Longa™, which have a high concentration of exosomes at the billion level. These exosomes significantly improve skin hydration and regeneration, as well as increase hair density and delay hair loss. They have the potential to encapsulate
and protect compounds of interest such as DNA, RNA, curcuminoids, flavonoids, terpenes, sugars, phytosterols, and organic acids. Furthermore, C. longa exosomes have shown involvement in intercellular communication by transporting specific growth factors, proteins, and peptides.14,15 Vytrus has also developed Centella
Reversa™, the concentrated secretome of totipotent cells from Centella asiatica cell cultures. This product contains a high billion- level concentration of exosomes rich in growth factors, proteins, and peptides. These Centella asiatica exosomes can activate patterns of expression of cutaneous genes related to ageing, the skin barrier, and hydration in keratinocytes, with powerful regenerative and anti-wrinkle effects on the skin. This combination of properties leads to the reversal of cell senescence.16 Vytrus’ exosome-based active ingredients
are highly effective for skin and hair and hold great potential for innovation in cosmetics. Their sustainable plant biotechnology
A Figure 1: Process of obtention of plant-derived exosomes in cell cultures
approach aligns with consumer demand for effective and eco-friendly skin and hair care products.
Biological activity The biological activity of plant-derived exosomes is a key factor in their effectiveness for skin and hair care. Understanding the production, isolation, visualization, and characterization of these exosomes is crucial to harness their full potential.
B The use of the transmission electron
microscope (TEM) enabled the acquisition of physical data such as the diameter and structure of the plant-derived exosomes. TEM images validated the structural integrity and uniformity of the exosomes, while nanoparticle tracking analysis (NTA) data demonstrated their high concentration and optimal size.
C D
Production and characterization of natural plant exosomes in Curcuma longa and Centella asiatica cell cultures Isolation and visualization of plant-derived exosomes through TEM & Cryo-TEM The isolation of plant exosomes from Centella asiatica and Curcuma longa cell cultures was achieved through a series of ultracentrifugation steps. Initially, cells were cultured under controlled conditions to optimize exosome production. The culture medium containing secreted exosomes was then collected and subjected to differential centrifugation, followed by ultracentrifugation at high speeds to pellet the exosomes based on their density . Transmission Electron Microscopy (TEM)
and cryo-TEM were utilized to visualize the isolated exosomes, providing detailed images of their structures and confirming their size and morphology. The exosomes from Curcuma longa,
Figure 2: Curcuma longa cell culture exosomes. Images of C. longa exosomes taken by transmission electron microscopy (TEM). At x10,000 magnification (A), the overall morphology of the exosomes is visible. At x20,000 magnification (B), more detailed structural features can be observed. At x30,000 magnification (C), finer structural elements are highlighted, and at x60,000 magnification (D), a highly detailed view of the exosome surface and internal structures is provided
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obtained under the production conditions of Capilia Longa and Turmeria Zen, displayed similar spherical vesicles with slightly larger average diameters. The Curcuma longa exosomes demonstrated uniform vesicles with a high concentration, indicative of successful isolation (Figures 2 and 3). TEM images obtained for the exosomes from Centella asiatica, obtained under the production conditions of Centella Reversa, revealed well- defined, spherical vesicles with consistent size distribution (Figure 4).
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