EXOSOMES
actual exosome count, making it difficult for consumers to gauge product authenticity. Scientific studies demonstrating exosome efficacy are typically conducted using purified, high-concentration exosomes. However, these concentrations rarely translate
into final formulations, where active amounts may fall significantly short of those used in research.
6. Formulation compatibility Exosomes degrade in harsh conditions and should not be included in surfactant, detergent, or alcohol-heavy formulations, as these will disrupt their lipid bilayer membrane. For the longest shelf life (two years at room temperature19
), they should be stored in a
lyophilized form and reconstituted to a delivery medium before application. Alternatively, they can be formulated into
stable serum or cream products, designed to maintain the integrity of the exosomes while delivering their benefits effectively.
7. Avoiding synthetic mimics Some products claim to use ‘synthetic exosomes’ or ‘exosome mimetics’ as a cost- effective alternative. While these may have potential applications, they will likely operate through entirely different modes of action compared to naturally derived exosomes.
8. Terminology clarity Exosomes may also be referred to as ‘extracellular vesicles’ or ‘vesicles’ in ingredient lists and scientific discussions. If the INCI list does not explicitly include the terms ‘exosomes’ or ‘vesicles’, it is unlikely that the product contains these components. The field’s growth underscores the need for
robust regulatory frameworks, which should include standardized testing to verify exosome concentration and quality, as well as mandatory disclosure of sourcing and characterization methods. Clear and enforceable guidelines will not only ensure product safety but also protect consumers from misleading claims, fostering trust and transparency in the cosmetics market.
The future of exosome cosmetics Exosomes and extracellular vesicles represent a breakthrough in skin care, combining cutting- edge biotechnology with natural efficacy. By sourcing exosomes directly from nature rather than lab-grown alternatives, Exolitus unlocks limitless development potential, allowing search for new active properties and modes of action to broaden portfolio. Furthermore, the natural origins of these
exosomes enable them to be blended synergistically, creating proprietary formulations that address multiple skin concerns holistically. Following the MISEV guidelines, Exolitus
ensures comprehensive characterization of exosomes, including their size, concentration, and bioactive content. Without this scientific rigour, the results in advanced skin care will not be possible. Exolitus strives to make exosome science
accessible and effective, bridging the gap between cutting-edge research and daily skin care solutions. Dedicated work needs to be put
www.personalcaremagazine.com e7
1.4 1.2 1.0 0.8 0.6 0.4 0.2 0
59
172
101
396
716 780
100 200 300 400 500 600 700 800 900 1000 Size (nm)
Figure 5: Cryo-TEM images (on top) and (right) NTA results forMelissa officinalis exosomes. The Cryo-TEM images reveal their lipid bilayer membrane and the inner void where bioactive cargo, such as proteins, lipids, and RNA, is stored. The accompanying NTA graph illustrates the particle size distribution and concentration of Melissa exosome
in to position exosomes and extracellular vesicles not as a passing trend but as a cornerstone of the cosmetics industry’s future.
PC
References 1. Harding CV, Heuser JE, Stahl PD. Exosomes: Looking back three decades and into the future. The Journal of Cell Biology. 2013; Feb 18;200(4):367–71
2. Thakur A, Shah D, Rai D, Parra DC, Pathikonda S, Kurilova S et al. Therapeutic Values of Exosomes in Cosmetics, Skin Care, Tissue Regeneration, and Dermatological Diseases. Cosmetics. 2023 Apr 1;10(2):65
3. Jeppesen DK, Zhang Q, Franklin JL, Coffey RJ. Extracellular vesicles and nanoparticles: emerging complexities. Trends in Cell Biology. 2023; Feb.
4. Welsh JA, Goberdhan DCI, O’Driscoll L, Buzas EI, Blenkiron C, Bussolati B et al. Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. Journal of Extracellular Vesicles. 2024; Feb 1;13(2):e12404
5. Mulcahy LA, Pink RC, Carter DRF. Routes and mechanisms of extracellular vesicle uptake. Journal of Extracellular Vesicles. 2014; Jan;3(1):24641
6. Zhang Y, Liu Y, Liu H, Tang WH. Exosomes: biogenesis, biologic function and clinical potential. Cell & Bioscience [Internet]. 2019; Feb 15;9(1)
7. Calzoni E, Bertoldi A, Cusumano G, Buratta S, Urbanelli L, Emiliani C. Plant-Derived Extracellular Vesicles: Natural Nanocarriers for Biotechnological Drugs. Processes. 2024; Dec 23 12(12):2938–8
8. Kim HI, Park J, Zhu Y, Wang X, Han Y, Zhang D. Recent advances in extracellular vesicles for therapeutic cargo delivery. Experimental & Molecular Medicine. 2024; Apr 1;1–14
9. Lee S, Jung SY, Yoo D, Go D, Park JY, Lee JM et al. Alternatives of mesenchymal stem cell- derived exosomes as potential therapeutic platforms. Frontiers in Bioengineering and Biotechnology. 2024; Sep 9;12
10. Lu L, Bai W, Wang M, Han C, Du H, Wang
N et al. Novel roles of bovine milk-derived exosomes in skin antiaging. Journal of Cosmetic Dermatology. 2023; Dec 17;23(4):1374–85
11. Rome S. Biological properties of plant- derived extracellular vesicles. Food & Function. 2019;10(2):529–38
12. Ornella Urzì, Gasparro R, Nima Rabienezhad Ganji, Alessandro R, Raimondo S. Plant-RNA in Extracellular Vesicles: The Secret of Cross- Kingdom Communication. Membranes. 2022; Mar 23;12(4):352–2
13. Woith E, Guerriero G, Hausman JF, Renaut J, Leclercq CC, Weise C et al. Plant Extracellular Vesicles and Nanovesicles: Focus on Secondary Metabolites, Proteins and Lipids with Perspectives on Their Potential and Sources. International Journal of Molecular Sciences [Internet]. 2021; Jan 1; 22(7):3719
14. Golan-Gerstl R, Elbaum Shiff Y, Moshayoff V, Schecter D, Leshkowitz D, Reif S. Characterization and biological function of milk-derived miRNAs. Molecular Nutrition & Food Research. 2017; Jul 31;61(10):1700009
15. Zhou Q, Li M, Wang X, Li Q, Wang T, Zhu Q et al. Immune-related microRNAs are abundant in breast milk exosomes. International Journal of Biological Sciences [Internet]. 2012; 8(1):118–23
16. Bae IS, Kim SH. Milk Exosome-Derived MicroRNA-2478 Suppresses Melanogenesis through the Akt-GSK3β Pathway. Cells. 2021; Oct 22;10(11):2848
17. Vaswani K, Peiris HN, Koh Y, Hill RJ, Harb T, Arachchige BJ et al. A complete proteomic profile of human and bovine milk exosomes by liquid chromatography mass spectrometry. Expert Review of Proteomics. 2021; Aug 3;18(8):719–35
18. Martini H, Passos JF. Cellular senescence: all roads lead to mitochondria. The FEBS Journal. 2022; Jan 30
19. Lu L, Han C, Wang M, Du H, Chen N, Gao M et al. Assessment of bovine milk exosome preparation and lyophilized powder stability. Journal of Extracellular Biology. 2024; Nov 1;3(11)
March 2025 PERSONAL CARE
Concentration (particles/ml)
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