SKIN MICROBIOME


C. granulosum, M. luteus, S. caprae and S. hominis are now part of the communication network.


The majority is not collaborating. Still, there


is no network. All collaborations happen in small islands between non-skin microbiota members. The skin microbiome is still not recovered. The amount of communication lines is 4000 (Figure 3). After the treatment with the test product


(containing 2% salicylic acid and 2% EPI) a complete recovery of collaboration and communication is observed (Figure 2C). All the important protective members are again working together. Even the rebellious C. acnes and


S. epidermidis are again part of the communication network. Again, they work together with Corynebacteria, other Cutibacteria, and with protective fungi such as Malassezia. The skin microbiome is back in balance as the complete network of 14,000 communication lines (Figure 3) is active again.


Result 2: Microbial population evolution Besides studying the collaboration between the micro-organisms, the abundance of each micro-organism has been monitored. The acne- prone skin has a high abundance of C. acnes. An average of 79% was determined. When the skin was treated for 28 days with the placebo (containing 2% salicylic acid) the average became 82%.


■ Placebo ■ 1% EPI


60.0% 40.0% 20.0% 0.0%


-20.0% -40.0% -60.0% -80.0%


42.0% 30.0% 1.7%


95


-25.0%


-25%


-61.0% C.acnes S.epidermidis Protective microbiota


Figure 8: Microbiome composition changes after four hours, compared to initial situation With salicylic acid alone the dominancy of


C. acnes did not significantly decline. However, when the salicylic acid was combined with 2% EPI, C. acnes is reduced back to a healthy abundance of 57% (Figure 4). Not only is the C. acnes is normalized, but also the microbial diversity is completely restored with the prebiotic. The diversity increased from 250 species to a healthy level of 430 species (Figure 5). The diversity increased by 72%.


Result 3: Gene expressions With metagenomics, gen expressions can also be determined. When the acne-prone skin is only treated with salicylic acid (placebo), possible pathogenic gene expressions in C. acnes are still detected (Figure 6). However, when salicylic acid is combined


with the prebiotic EPI, only possible protective gene expressions are detected. Examples of some pathogenic behaviour and protective


www.personalcaremagazine.com


September 2023 PERSONAL CARE


Abundance change in %


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52  |  Page 53  |  Page 54  |  Page 55  |  Page 56  |  Page 57  |  Page 58  |  Page 59  |  Page 60  |  Page 61  |  Page 62  |  Page 63  |  Page 64  |  Page 65  |  Page 66  |  Page 67  |  Page 68  |  Page 69  |  Page 70  |  Page 71  |  Page 72  |  Page 73  |  Page 74  |  Page 75  |  Page 76  |  Page 77  |  Page 78  |  Page 79  |  Page 80  |  Page 81  |  Page 82  |  Page 83  |  Page 84  |  Page 85  |  Page 86  |  Page 87  |  Page 88  |  Page 89  |  Page 90  |  Page 91  |  Page 92  |  Page 93  |  Page 94  |  Page 95  |  Page 96  |  Page 97  |  Page 98  |  Page 99  |  Page 100  |  Page 101  |  Page 102  |  Page 103  |  Page 104  |  Page 105