UPCYCLED INGREDIENTS
Discussion Characterization of three different extracts of early-stage apples, obtained via Hyperfrequency-assisted extraction, shows the impact on the concentration of molecules that are targeted for this study: the inclusion of 33% of ethanol in the system, provides a higher concentration of Phlorizin in comparison with extraction 100% water and 50%/50% water/ ethanol. In the case of chlorogenic acid, the
extraction gives better results when the process is performed 100% in water. However, the fact that the formulation A1, 100% water extraction, can lead to sedimentation constraints, shows that for scale up and then, industrial applications of an upcycled early- stage apple extract, the suggestion is to work with formulation C1, 33% ethanol-extraction. Performed in vitro tests in formulation C1,
shows antioxidant and good indication for moisturizing benefits of this extract, which can be linked to the polyphenol content that Hyperfrequency technology helps to reach when upcycled early-stage apples are treated. These in vitro results fit with benefits that consumers seek when they are choosing well- aging products.
Conclusion The application of Hyperfrequency-assisted technology supports the valorization of side- stream, in this case, early-stage apples coming from the food industry. Physical characterization, molecules analysis
and in vitro evaluations show that one of the extracts delivered by this study meets all the parameters to be used safely in the cosmetic industry.
Acknowledgments The authors would like to acknowledge the Symrise Cosmetic Ingredients team.
PC
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-2.0 0.0 5.0 10.0 Min Figure 4: Chromatogram for determination of Chlorogenic acid in trial C1
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** ** ** 15.0 20.0 25.0
0.1
0.5 Early stage Apple Extract C1 (%)
Figure 5: Antioxidant capacity (%) depends on the concentration of early-stage apple extract. * p<0.05. ** p< 0.01 *** p< 0.001
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* *** ** 0.01 0.10 Early stage Apple Extract C1 (%)
Figure 6: ROS reduction (%) measured in fibroblast cells, depends on the concentration of early-stage apple extract. * p<0.05 ** p< 0.01 *** p< 0.001
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Figure 7: Hyaluronic acid modulation (%) measured in cell lysates, depends on the concentration of early-stage apple extract. * p<0.05 ** p< 0.01 *** p< 0.001
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* * *
1.00
0.005
0.01 Early stage Apple Extract C1 (%)
0.05
ROS Reduction (%)
mAU Antioxidative Capacity1 (%)
4.107 Chlorogensaure Hyaluronic acid modulation (%)
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