SILICONES 150 **** 100 50
200 150 100 50 0
0 Control Control + UVA
Figure 11: UVA-induced cytotoxicity in HaCaT keratinocytes. Bar graph representing cell viability levels normalised to control, after exposure of human HaCaT keratinocytes to UVA light (15 J/cm2
). Asterisks over bars indicate statistical significance versus control (unpaired student’s T-test)
the sample at the tested doses did not show any cytotoxicity effect. According to the test results, Nano Olive Oil is a natural antioxidant that can protect hair from harmful effects such as sunlight, pollution, cosmetic treatments, grooming practices. Studies on silicone and polyquaternium
show that these are two commonly used ingredients in skin care products, hair care products, anti-ageing creams etc. However, there have been some studies to show harmful effects of silicon on human health. According to research by Krystyna Mojsiewicz-Pieńkowska,2
silicones belonging
to the cyclo-siloxane group including cyclotetrasiloxane, cyclopentasiloxane, cyclohexasiloxane can cause damage to the skin’s protective film, leading to irreversible damage to the stratum corneum. Silicone is also harmful to the environment.
The European Chemicals Agency (ECHA) has classified cyclotetrasiloxane and cyclopentasiloxane as very difficult to degrade in the environment and very bio- accumulative.3
In 2015, the UK submitted **** 3000 2000 1000 0 0 Control C + UVA
Figure 13: UVA-induced oxidative stress in HaCaT keratinocytes. Bar graph representing ROS levels normalised to control, after exposure of human HaCaT keratinocytes to UVA light (15 J/cm2
). Asterisks over bars indicate statistical significance versus control (unpaired student’s T-test)
www.personalcaremagazine.com
Figure 14: Protective effect of Nano Olive Oil from the UVA-induced oxidative stress: Bar graph representing ROS levels normalised to control + UVA, after 24 hours of treatment in human HaCaT keratinocytes with Nano Olive Oil and subsequent exposure to with UVA light. Asterisks over bars indicate statistical significance versus control + UVA (Dunnett’s test)
November 2022 PERSONAL CARE 150 **** 100 50 *
Figure 12: Cell viability effects upon UVA exposure in Nano Olive Oil pre-treated cells. Bar graph representing cell viability levels normalised to control + UVA, after 24 hours of treatment in human HaCaT keratinocytes with Nano Olive at different concentrations followed by irradiation with UVA light at 15 J/cm2
. Asterisks over bars indicate statistical significance versus control (Dunnett’s test)
documents to ECHA to demonstrate the environmental risks posed by the use of silicon when discharged into wastewater.4 Polyquaternium is a conditioning agent, adding shine to the hair, but it is not biodegradable and is toxic to some aquatic species.5 Nano Olive Oil is extracted from natural
ingredients, safe and friendly to environment has the potential to completely replace or reduce the use of silicone and polyquaternium in shampoo while maintaining product effectiveness. This is a practical solution to decrease the
amount of silicone and polyquaternium waste in the environment, reducing the impact on the natural environment. Shampoo using Nano Olive Oil increases hair shine, reduces combing force, increases hair smoothness, and restores hair compared to regular dimethicone and polyquaternium-7 shampoos.
References 1. Hai Lai N, Thi Dang Hong N. Process for production of nano-microemulsion system of plant oil triglycerides. Wakamono
Corporation patent filed 16 August 2020. United States; US20200346174A1
2. Mojsiewicz-Pieńkowska K, Stachowska E, Krenczkowska D, Bazar D, Meijer F. Evidence of Skin Barrier Damage by Cyclic Siloxanes (Silicones)—Using Digital Holographic Microscopy. International Journal of Molecular Sciences. 2020;21(17):6375. doi: 10.3390/ijms21176375
3. European Chemical Agency. Identification of PBT and vPvB substance. 2012.
https://echa.europa.eu/documents/10162/ 13628/octamethyl_pbtsheet_ en.pdf/19d40ef0-a0d2-4e93-8840- ae1e1572e5ca
4. European Chemical Agency, Annex XV restriction report, 2015.
https://echa.europa. eu/documents/10162/9a53a4d9-a641- 4b7b-ad58-8fec6cf26229
PC
5. Cumming J, Hawker D, Chapman H, Nugent K. The Fate of Polymeric Quaternary Ammonium Salts from Cosmetics in Wastewater Treatment Plants. Water, Air and Soil Pollution. 2010;216(1-4):441-450. doi: 10.1007/s11270-010-0543-5
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65
Relative ROS levels (%)
Relative ROS levels (%)
Relative ROS levels (%)
Relative ROS levels (%)
C
C + UVA 0.0001% + UVA 0.001% + UVA
0.01% + UVA
C
C + UVA 0.0001% + UVA 0.001% + UVA
0.01% + UVA
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