ANTI-POLLUTION
Multiethnic active for exposome protection
Consuelo García, Eva Gómez, Ramón Subirós, Sandra Méndez, Albert Soley, Gemma Mola, Núria Almiñana, Raquel Delgado – Lipotec ABSTRACT
Extreme weather events, like heat waves, droughts, and wildfires, combined with the increase in aggressors like air pollution, are preventing many consumers from enjoying the great outdoors. Lipotec has discovered a new, natural active biotech ingredient, Xpozuki™, which is able to protect the skin, scalp, and hair against the exposome. On the skin and scalp, it can protect against oxidative damage, and on the hair fibre, it can protect against protein carbonylation and lipid peroxidation. In clinical trials, the new active ingredient sourced from organic azuki beans has demonstrated the ability to minimise skin and hair damage induced by exposome stress on a diverse group of female panelists with various skin and hair types, improving skin tone and texture homogeneity, as well as hair vitality. In doing so, the new active ingredient, which harnesses biotechnological expertise to enrich the content of Azukides natural peptides, can help to support all consumers to feel more protected against environmental aggressors.
With the rapid change in environmental conditions, consumers are growing more concerned about not only the impact of pollution on human health, but also the impact of other environmental exposures, too. In 2005, the term ‘exposome’ was coined by the epidemiologist Christopher P. Wild to refer to the lifelong totality of exposure to environmental and lifestyle factors. Later, the concept would be adapted to
dermatology, which marked a shift in the way scientists viewed the impact of environmental factors on the skin.1
aggressors was combined, like air pollutants and UV radiation, or temperature and UV radiation,4
Until that point, the impact of
these factors had been studied separately, and molecular crosstalk was poorly understood.2,3 Yet when the impact of several environmental
the results could not be explained
by the cumulative effect of each of the factors separately. This suggested that there may be synergistic effects and molecular crosstalk, which could only be elucidated through an exposome approach. Here, we report on the first study in the cosmetic field to look at the combined effects
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of UV radiation, air pollution, and greenhouse damage, as well as pollen, on the skin, scalp, and hair. We also discovered our Xpozuki biotech ingredient, hereafter known as the natural active ingredient, can protect, defend, and repair damage caused by these exposome factors, which can be used in leave-on and rinse-off cosmetic applications.
Preserving and repairing skin and scalp cell viability against exposome damage Human dermal fibroblasts (HDFa) and human epidermal keratinocytes (HEKa) coculture cells were seeded in 96-well plates in culture medium. After 24 hours of incubation at 37˚C in 5% CO2
humidified air, cell culture medium was
removed, and fresh medium was added with natural active ingredient solubilised at 1% or 2% (v/v) for one hour. Then, cells were exposed to PM2.5 (Diesel
particulate matters NIST1650b) followed by solar irradiation for two hours, in presence of the natural active ingredient. After solar irradiation, cells were incubated at 38°C in 20% CO2
humidified air for two hours (greenhouse
stress damage), in presence of the natural active ingredient. Control cells were maintained at room
temperature and protected from light during the irradiation time and were incubated at normal conditions 37°C in 5% CO2
humidified air. Just
after stress exposure, cell viability was quantified following PrestoBlue reagent instructions. To study if our natural active ingredient could
help HEKa-HDFa cells to repair from exposome damage, coculture cells were seeded and,
after 24 hours of incubation at 37°C in 5% CO2 humidified air, cells were exposed to PM2.5 followed by solar irradiation for two hours. After solar irradiation, cells were incubated humidified air for 22 hours
at 38°C in 20% CO2
(greenhouse stress damage). Control cells were maintained at room temperature and protected from light during the irradiation time and then were incubated at normal conditions, 37°C in 5% CO2
humidified air. On the next day, cells were washed and
treated with natural active ingredient at 1% and 2% (v/v) in culture medium for six hours. Then, cell viability was quantified following PrestoblueTM
reagent instructions. November 2022 PERSONAL CARE
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