64 SILICONES
Cell viability (resazurin) Regarding cell viability, the exposure of HaCaT cells to UVA light at 15 J/cm2
TEST 1: Samples
induced a
significant decrease in this parameter by 40.7 ± 6.3%, compared to the untreated control, thus confirming the deleterious effects of the irradiation protocol in our cellular system (Figure 11). In addition, upon pre-exposure of cells
to Nano Olive Oil for 24 hours, there was a protective trend against UVA-induced cytotoxicity, with the maximum effect reached at the highest dose tested, 0.01%. In this regard, cells treated with the tested
sample at this concentration displayed an increase in cell viability by 24.9 ± 14.2% versus untreated control + UVA, although results did not reach statistical significance (p > 0.05) (Figure 12).
Oxidative stress after UVA Results showed that UVA light at 15J/cm2 significantly increased ROS levels in HaCaT keratinocytes by 2556 ± 228.1%, compared to the untreated control, thus confirming that the UVA irradiation protocol was effectively inducing oxidative stress in the cells (Figure 13). In agreement with the protective effects
suggested by the cell viability data, results obtained from cells pre-treated with Nano Olive Oil for 24 hours indicated that this product at 0.01% significantly reduced ROS levels by 36.9 ± 12.7%, compared to the untreated control + UVA. In the same way, the same product at 0.001%
and 0.0001% reduced average ROS levels by 14.6 ± 12.7% and 7.2 ± 12.7%, respectively, although these results did not reach statistical significance (p > 0.05) (Figure 14). In conclusion, the in vitro treatment
with Nano Olive Oil - 20 % in human HaCaT keratinocytes, exhibits antioxidant and UVA- protective capabilities, substantiated in a significant reduction of ROS levels and a non- significant prevention of cell viability decrease at 0.01% concentration, compared to the untreated, UVA-exposed control. Additionally, results indicated the sample at the tested doses did not show any cytotoxicity effect.
1
2 3 4 5 6
Contents Purified water
Purified water 25g + Nano Olive Oil 1g Purified water 25g + Argan Olive Oil 1g Yuzu Oil 25g + Nano Olive Oil 1g MCT Oil 25g + Nano Olive Oil 1g Dimethicone 25g + Nano Olive Oil 1g
Completely dissolved Layer separation Dissolved Dissolved Dissolved
Remarks
Figure 9: Solubility of samples: (1) Purified water; (2) Purified water 25g + Nano Olive Oil 1g; (3) Purified water 25g + Nano Olive Oil 1g; (4) Yuzu Oil 25g+ Nano Olive Oil 1g; (5) MCT Oil 25g+ Nano Olive Oil 1g; (6) Dimethicone 25g + Nano Olive Oil 1g
Conclusion Based on Tests 1 and 2 performed in independent laboratory Daebong Life Science, Nano Olive Oil shampoo improves hair gloss, smoothness and reduces friction better than shampoo containing dimethicone (silicone). This means that Nano Olive Oil can replace dimethicone in shampoo production, ensuring the requirements of consumers, and solving the disadvantages caused by abuse of Silicone to humans and the environment. school.
In addition, the results in Test 3-6 showed
that Nano Olive Oil shampoo brought a good feeling to the hair on the survey of volunteers, curl retention, increasing the hair strength, hair gloss and reduced brushing force compared to shampoos containing Polyquaternium-7. The Nano Olive Oil ingredient is replaceable, reducing the use of Polyquaternium-7 while still meeting today’s consumer standards for shampoo products. The effectiveness of shampoos containing
Nano Olive Oil to treat damaged hair surface and split ends was demonstrated in Tests 7 and 8. Electron microscope images show damaged/split hair recovered over one treatment. The test results are consistent with recent scientific studies in the cosmetic industry, especially the application of natural olive oil to shampoo products. The applicability of Nano Olive Oil was
proved to be stable, uniform, and completely soluble in both water and oil systems in Test 9. Nano Olive Oil microemulsions created from ‘Process for producing microemulsion system of Nano essential oil’ can be used as shampoo, with potential to expand the application in the cosmetic industry. In the above test, it is shown that Nano Olive Oil has compatibility and easily dissolves in water which is the preeminent property in industrial scale production. The antioxidant and UVA protection
Figure 10: Representative image of HaCaT cells in culture. Bright field optical microscopy picture of cultured HaCaT cells at 80 % confluence. This is the typical stage of a HaCaT culture before trypsinization and subculture or cell seeding for subsequent experiments
PERSONAL CARE November 2022
capabilities of Nano Olive Oil in Test 10 were substantiated by a significant reduction of ROS levels compared to the untreated, UVA- exposed control. Additionally, results indicated
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