ANTI-AGEING 17


Figure 9: Activity on stress. Mitochondria Membrane Potential is


directly related to mitochondria activity; the higher the mitochondria activity, the higher the membrane potential (hyperpolarised membrane).


Protection mode First, the activity of Targeted Q10 to prevent the extra damage induced on aged fibroblasts by UV-A radiation was evaluated, assessing the variation of mitochondrial membrane potential as well as ROS level. Mitochondria membrane potential has


been evaluated by JC-1 assay; variations of the mitochondrial membrane potential can be detected by JC-1 dye. When mitochondrial membrane depolarises, this dye is in the form of green-fluorescent monomer. Conversely, in the case of hyperpolarised membrane, the dye is in the form of red-fluorescent J-aggregates. Briefly, the study was performed using senescent NHDF cells. NHDF cells have been incubated with Targeted Q10 at 0.25% and free Q10 at 0.00005% (this concentration is equivalent to the amount


of Q10 in Targeted Q10 at 0.25%, since Trojan Q10 contains 0.02% of Q10) for 48 hours, and then irradiated with UV-A (3 J/cm2


). Afterwards, JC-1 solution was


added, and images have been taken as well as fluorescence was measured. The images shown in Figure 9 refer


6/8/20 15:59


respectively to the not-treated fibroblasts, fibroblasts treated with UV-A, fibroblasts previously treated with Free Q10 and then exposed to UV-A and fibroblasts previously treated with Targeted Q10 and then exposed to UV-A. Cells nuclei are stained in blue with


September 2020


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