16 ANTI-AGEING


membrane. All these steps are represented in Figure 6. They are very important and critical for an efficient mitochondrial delivery. In order to assess that Targeted Q10 is


able to be up-taken by fibroblasts, Normal Human Dermal Fibroblasts (NHDF) have been incubated with 0.1% Rhodamine- labelled Targeted Q10 (red colour). The incubation time was 1 hour. Cells nuclei have been stained with DAPI (blue colour) and NHDF cytosol with Mitotracker (green colour). NHDF cells incubated with Targeted Q10 have been analysed using a Leica Confocal Microscopy (see Figure 7). From the images on the top, it can be observed the first step where Trojan particles attach to the cellular membrane and, afterwards, internalise. In fact, we can notice from the merge of the three microscope channels that Trojan particles (red) are preferentially localised on cells membrane. Later, from the images on the bottom, the internalisation process takes, where Trojan nanoparticles (red coloured pixels) are distributed along cellular cytosol. We can conclude that Targeted Q10 shows high affinity to fibroblasts and it is quickly cellular up-taken by them. Now that Targeted Q10 particles are


inside fibroblast, they have to find their way to the mitochondria. We have further investigated Targeted Q10 internalisation into fibroblasts, and in particular its preferential localisation at mitochondria level. NHDF have been incubated with 0.1% Rhodamine-labelled Targeted Q10 (red colour), for 45 min. Cellular mitochondria have been stained with Mitotracker (green colour). NHDF cells incubated with Targeted Q10 have been analysed by a Leica Confocal Microscope


Figure 7: Uptake into fibroblasts.


and the images are shown in Figure 8. As previously seen, Targeted Q10 is quickly internalised by fibroblasts and from Figure 8 it can be seen co-localisation between Targeted Q10 (red colour) and mitochondria (green colour). This further supports the hypothesis that Targeted Q10 not only it is able to be quickly internalised by fibroblasts but also preferentially localised at mitochondria level.


Activity on mitochondria As previously discussed, it is well-known that mitochondria are the first responsible of ROS production and that the level of ROS progressively increases with ageing, while ATP levels decrease. The described scenario becomes even more dramatic when cells undergo stress induced by


external factors such as UV radiation. In fact, cells counteract to the induced damage by activating high-energy demanding pathways, which require extra energy and increase mitochondrial activity. However, since these mitochondria are not properly working, instead of generating extra energy, they are only generating extra mROS. When Q10 is effectively delivered to


mitochondria, it has the potential of “protect” mitochondria from ROS induced damage as well as to “re-energise” aged mitochondria. In order to evaluate this potential, multiple studies have been performed and we have evaluated the impact of Targeted Q10 on mitochondria activity in terms of: 1.Mitochondrial Membrane Potential 2.ROS level


Figure 8: Localisation at mitochondrial level. PERSONAL CARE EUROPE September 2020


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52  |  Page 53  |  Page 54  |  Page 55  |  Page 56  |  Page 57  |  Page 58  |  Page 59  |  Page 60  |  Page 61  |  Page 62  |  Page 63  |  Page 64  |  Page 65  |  Page 66  |  Page 67  |  Page 68  |  Page 69  |  Page 70  |  Page 71  |  Page 72  |  Page 73  |  Page 74  |  Page 75  |  Page 76  |  Page 77  |  Page 78  |  Page 79  |  Page 80  |  Page 81  |  Page 82  |  Page 83  |  Page 84  |  Page 85  |  Page 86  |  Page 87  |  Page 88  |  Page 89  |  Page 90  |  Page 91  |  Page 92