ANTI-AGEING 15
able to find its way to mitochondria. In fact, the second peptide, D-Arginyl Tyrosinyl Ornithinyl Phenylalanine, is a mitochondria- penetrating peptide, which sequence has been designed for promoting the positive interaction of Trojan Q10 with the mitochondrial membranes. When Trojan Q10 particles are localised at mitochondrial level, they degrade and release Q10. This can take part to mitochondria respiration, increasing mitochondria activity and ATP production as well as effectively neutralising mROS. Worthy of attention is that Trojan Q10 ,now referred to as 'Targeted Q10', contains only 0.02% of CoQ10.
A long journey to mitochondria First of all, Trojan particles need to properly penetrate into the skin and so reach the dermis, and in particular, the fibroblasts. In order to demonstrate that Trojan particles are effectively able to provide a better penetration than that of the free active ingredient, we have performed a skin penetration study with pig skin assembled on a Franz cell. In Figure 4, the collected confocal images of the pig-skin loaded with red-labelled Trojan particles are shown. Cells nuclei have been stained in blue. When the active ingredient is encapsulated in Trojan particles, the penetration is faster and more effective, leading to zero-waste of the product: after only 2 hours, Trojan is localised at the dermis level (red pixels) and it penetrates to deep layers over time. As shown in Figure 5, we can also build
up a kinetics profile of penetration and perform a semi-quantitative analysis. In
Figure 5: Trojan technology kinetics.
particular we can observe the kinetics penetration profile of Trojan vs. that of the free active on the left side of the Figure 5. A faster penetration is observed in the case of Targeted Q10. After 2 h, the 60% of the active penetrates the skin and after 6 h, almost the 90% has already penetrated. Moreover, in the case of Targeted Q10, the 100% of the product loaded in the Franz cell is collected in the receptor compartment after 20 h (100 % vs. 35% of free rhodamine). On the right side of Figure 5 we can also observe the kinetic profiles of penetration of Trojan in epidermis and dermis. By Targeted Q10, it is possible to achieve a fast and deep penetration of the active up to the dermis level. After only 2hr, 70% of Targeted Q10 is localised at dermis
level, and after 10 h from the application, 90% has reached the dermis. Now that Trojan particles have reached
the dermis, they have to be internalised by fibroblasts and then to be localised at mitochondria level. Mitochondria-targeting and delivery is very challenging, because of the numerous barriers the product needs to cross. In fact, in order to reach the mitochondria, the product needs (i) first, to reach the fibroblast, cross their cellular plasmatic membrane and being up-taken by them; (ii) then the product needs to travel within their cytosol and be directed to the mitochondria (cytosol trafficking); (iii) when finally arriving on the mitochondria, it needs to be adsorbed on the surface and release Q10 through mitochondria double
Figure 6:Mechanism steps. September 2020 PERSONAL CARE EUROPE
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