BIOTECHNOLOGY A
200 180 160 140 120 100 80 60 40 20 0
CRE * * * * o
α-B(µM): α-MSH:
Fk: - + + - + + + - - - - - - - H-89: - - + + - - - - + - - - - - - - - - 1 10 50 - - 1 10 50 1 10 50
- -
- - - - - - - + + + + + - - - o o o OH H B
10 8 6 4 2 0
* * o * * o o C
800 700 600 500 400 300 200 100 0
α-B(µM): α-MSH:
Fk: - + + - + + + - - - - - - - H-89: - - + + - - - - + - - - - - - - - - 1 10 50 - - 1 10 50 1 10
- - - - - - - + + + + + - - Figure 6: α-Bisabolol inhibits melanin production by blocking cAMP signaling pathway
shown in Figure 6d, CREB phosphorylation was also inhibited by α-Bisabolol. The above indicated that α-Bisabolol operates upstream of the cAMP production step.10
Sustainable biotechnology to achieve large-scale production of
high-purity α-Bisabolol Based on the current situation, there are still some deficiencies in the production process of α-Bisabolol. For example, α-Bisabolol from synthetic sources has a racemic structure, and the purity is as low as about 85%. α-Bisabolol from natural sources is easily
limited by raw materials. In order to optimize the carbon footprint and promote global biodiversity, Aneco has achieved large-scale production of this product through R&D innovation and advanced sustainable biotechnology, and has developed a physiologically active, single- configuration (-)-α-Bisabolol BioSyn-Bisabolol has a purity of up to 99%. At the same time, in order to investigate the
stability of the fermented α-Bisabolol ingredient under the conditions of high temperature of 60°C, high humidity of RH92.5%, and strong light irradiation (illuminance 4500lx±500lx), we conducted a series of accelerated tests according to the Chinese Pharmacopoeia 2020. Conditions and selection of suitable packaging materials are provided for reference. The results showed that compared with
the results of the Day 0 test, the properties and purity of each test index did not change significantly, as shown in Figure 7 and Figure 8.
Conclusion BioSyn-Bisabolol, developed by Aneco using sustainable biotechnology, has a single configuration and 100% bio-based carbon, which solves the problems of sustainability and stable
100 97.5 95
92.5 90
High temperature High humidity Light Contrast
α-B(50µM): I.t(min):
α-MSH: - -
- +
+ -
+ +
- - - +
+ -
D 1 * *
0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0
+ - + -
- +
+ + - +
0 0 0 0 15 15 15 15 30 30 30 30 α-MSH: α-B(50µM):
-P-CREB CREB
- -
- +
* *
99
*
1 +
10 50 +
-
1 -
10 50 -
-
Figure 7: Appearance changes of BioSyn-Bisabolol under different conditions
supply. The purity can reach more than 99% is also one of its highlights. Under the conditions of high temperature
of 60°C, high humidity of RH92.5%, and strong light irradiation (illuminance of 4500lx±500lx), the fermented α-Bisabolol ingredient can also maintain a certain stability. Therefore, it has a huge market prospect in the
cosmetic industry, especially in the application of sensitive skin. At the same time, upgrading more products through innovation and achieving the greatest ecological application effect is also the goal of our continuous efforts.
References 1. Li An, Yang Yuanyuan. Research progress in the diagnosis and classification of sensitive skin. Chinese Cosmetics. Z3(2022):114-118
2. He Li et al. Expert consensus on the diagnosis and treatment of sensitive skin in China. Chinese Journal of Dermatology and
High temperature ■ High humidity■ Light ■
Venereology. 31.01(2017):1-4. doi:10.13735/j. cjdv.1001-7089.201611168
3. Zhao Xiaomi. This skincare race has grown by 1000%, with the Michel brand leading the way. Chinese Cosmetics. 02(2023):64-66
4. Yu Mingying et al. Research progress on the mechanism of sensitive muscle formation and anti sensitive components. Daily Chemical Science. 44.06(2021):32-37+52
PC
5. Baylac S, Racine P. Inhibition of 5-lipoxygenase by essential oils and other natural fragrant extracts. International Journal of Aromatherapy. 13.2-3 (2003): 138-142
6. Maurya AK et al. α-(-)-bisabolol reduces pro-inflammatory cytokine production and ameliorates skin inflammation. Current Pharmaceutical Biotechnology. 15.2 (2014): 173-181
7. Seungbeom et al. Inhibitory effects of (-)-α-bisabolol on LPS-induced inflammatory response in RAW264. 7 macrophages. Food and Chemical Toxicology. 49.10 (2011): 2580-2585
8. Van Zyl RL et al. The biological activities of 20 nature identical essential oil constituents. Journal of Essential Oil Research.
18.sup1 (2006): 129-133
9. Braga PC et al. Antioxidant activity of bisabolol: inhibitory effects on chemiluminescence of human neutrophil bursts and cell-free systems. Pharmacology. 83.2 (2009): 110-115
0d 5d Time
www.personalcaremagazine.com Figure 8: Purity trend of BioSyn-Bisabolol under different conditions
10d 30d
10. Kim S, Lee J, Jung E, Huh S, Park JO, Lee JW, Byun SY, Park D. Mechanisms of depigmentation by alpha-bisabolol. J. Dermatol. Sci. 2008 Dec;52(3):219-22
October 2023 PERSONAL CARE
Relative luciferase activity Purity %
Melanin content (% of control)
cAMP levels (pmol/7x105
cells)
Levels of P-CREB (P-CREB/CREB)
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