60 ANTI-AGEING
Alzheimer’s disease (AD) patient were used as a cellular model for sleep deprivation. Treatment of AD-fibroblasts with 0.01 % Iodobacter ssp. extract significantly increased ATP levels by 27.5 % after 120 minutes compared to untreated cells (Fig 2). Moreover, the number of MAM contact points, which was increased in AD fibroblasts compared to healthy control cells, significantly decreased upon treatment with an extract of Iodobacter ssp. (Fig 3 and 4). Together, these results show that an
extract of Iodobacter ssp. alleviates signs of cellular stress and supports the functions of both the mitochondria and the ER for more efficient protein folding despite ageing and lack of sleep.
Amelioration of visible signs of tiredness skin in a mixed study population The efficacy of the Iodobacter ssp. extract was further assessed in a placebo- controlled clinical study including female and male Caucasian volunteers with a tired appearance marked by dark circles around the eyes and medium-deep crow’s feet. Following application of a cream containing 2% Iodobacter active for 14 days, wrinkle depth of crow’s feet was significantly reduced by 15.9 % compared to initial conditions (Fig 5). Moreover, clinical-grade evaluation confirmed a significant reduction of visible facial tiredness compared to initial conditions, which was observed in 71% of the volunteers (Fig 6). The reduction in wrinkle depth as well as the improvement of tiredness were also visible in photographs of male and female volunteers (Fig 7). A second, placebo-controlled clinical
Before *p<0.05 versus initial conditions 100 n Placebo n 2% IceAwakeTM
75
* 50
25
0 Figure 7: Visible improvement of wrinkle depth and dark circles with IceAwakeTM
study with overworked Asian volunteers with little and bad quality sleep confirmed the significant reduction of wrinkle depth following 14 days of treatment with a cream containing 2% Iodobacter active (data not shown). Additionally, skin radiance significantly increased by 9.2% compared to initial conditions (Fig 8).
Overall, these data demonstrate that after just 14 days of application, Iodobacter ssp. extract rejuvenates tired skin by reducing wrinkle depth and visible signs of tiredness as well as increasing skin radiance.
Conclusion
Using the extract of Iodobacter ssp., the novel active ingredient IceAwakeTM
was
developed. Targeting a recently discovered aging mechanism based on protein folding and chaperone activity, treatment of stressed cells with the extract improved the
After 14 days .
ER stress response in vitro. Clinical studies proved a positive effect on tired and stressed skin. Regular application leads to a visible skin rejuvenation and an increase in radiance despite a hectic lifestyle.
PC
References 1 Walter P, Ron D. The Unfolded Protein
Response: From Stress Pathway to Homeostatic Regulation. Science. 2011; 334(6059):1081-1086
2 Gething MJ. Role and regulation of the ER chaperone BiP. Semin Cell Dev Biol. 1999; 10(5): 465-472
3 N Naidoo et al. Aging Impairs the Unfolded Protein Response to Sleep Deprivation and Leads to Proapoptotic Signaling. J Neurosci. 2008; 28(26):6539-6548
4 Pickard A et al., Preservation of circadian rhythms by the protein folding chaperone, BiP. FASEB J. 2019; 33(6): 7479-7489
5 Naidoo N et al. Sleep deprivation induces the unfolded protein response in mouse cerebral cortex. J Neurochem. 2005; 92(5):1150-1157
6 Naidoo N et al. Aging and sleep deprivation induce the unfolded protein response in the pancreas: implications for metabolism. Aging Cell. 2014; 13(1): 131-141
7 RC Taylor, Aging and the UPR(ER). Brain Res. 2016; 1648(Pt B): 588-593
8 van Vliet AR, Agostinis P. Mitochondria- Associated Membranes and ER Stress. Curr Top Microbiol Immunol. 2018; 414: 73-102
Before After 14 days
9 Chikahisa S, Séi H. The role of ATP in sleep regulation. Front Neurol., 2011; 2: 87
10 Russell NJ. Psychrophily and resistance to low temperature. In: C Gerday and N Glansdorff (eds) Extremophiles (Life under extreme environmental Condition), Encyclopedia of Life Support Systems (EOLSS) Developed under the Auspices of the UNESCO. Eolss Publishers, Oxford, UK.
11 Zakhia F et al. Cyanobacteria in Cold Ecosystems. In: R Margesin et al., Psychrophiles: from Biodiversity to Biotechnology. Springer-Verlag Berlin Heidelberg, 2008; Chapter 8, 121-135
Figure 8: Increase in radiance with IceAwakeTM PERSONAL CARE ASIA PACIFIC .
12 Naidoo N. Cellular stress/the unfolded protein response: Relevance to sleep and sleep disorders.
November 2019
Volunteers showing visible improvements of tiredness in %
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92 |
Page 93 |
Page 94 |
Page 95 |
Page 96 |
Page 97 |
Page 98 |
Page 99 |
Page 100 |
Page 101 |
Page 102 |
Page 103 |
Page 104 |
Page 105 |
Page 106 |
Page 107 |
Page 108 |
Page 109 |
Page 110 |
Page 111 |
Page 112 |
Page 113 |
Page 114 |
Page 115 |
Page 116 |
Page 117 |
Page 118 |
Page 119 |
Page 120
