MEN’S GROOMING 41 Mean fluorescence intensity emitted by total ROS
n Control n UVA/UVB n MRS (0.5%) n MRS (1%) n MRS (2.5%)
SOD activity (mU/mg of proteins)
n Control n UVA/UVB n MRS (0.5%)+UVA/UVB n MRS (1%)+UVA/UVB n MRS (2.5%)+UVA/UVB
-18%
-27% -24%
T0 T30min Figure 5: Study of ROS. Induction by UVA/UVB.
that at concentrations of 0.5%, 1% and 2.5%, an increase of water retention in the dehydrated epidermis was apparent at T0+15 min respectively by 24%, 30% and 35% ; and at T0+30 min, respectively by 29%, 36% and 43% compared to non- treated controls.
In the study of dynamic water at concentrations of 0.5%, 1% and 2.5%, a decrease of the trans-epidermic transfer of the [3
H]-H2
O was shown at T0+15min respectively by 28%, 32% and 38% ; at T 0+30 min respectively by 26%, 29% and 34 % ; and at T 0+60 min, respectively by 20%, 23% and 29%, compared to non-treated controls.
Strengthening natural antioxidant protection in the epidermis Oxidation (due to numerous environmental factors, such as UV and pollution, and internal factors such as the metabolism and stress) is a general phenomenon that affects all skin cells, damaging the components of their structure. However, the production of free radicals attacks our skin in different ways: oxidising cell membranes (lipid peroxidation), and proteins generally, ultimately damaging the cell DNA. Naolys decided to verify the sequoia plant cells’ activity in terms of the overall production free radicals (Fig 5), and also its action on the two main antioxidant enzymes: SOD (superoxide dismutase) (Fig 6) and catalase.
SOD accelerates the transformation of superoxide ions into hydrogen peroxide
Study of the inflammation mediators IL1-alpha (pg/ml) IL-6 (pg/ml) -18% -21% -28% +30% -24% PEG2 (pg/ml) -17% +27% -26% +29% -26%
-33% -29%
T3h T0 T30min Figure 6: Study of the SOD activity. Induction by UVA/UVB.
which catalase then converts into water and oxygen, thereby preventing the formation of carbon dioxide in the blood and also the formation of toxins and mutations in the DNA. In the study of total ROS at
concentrations of 0.5%, 1% and 2.5%, there is a decrease of the reactive oxygenated species (ROS) after 30 min of irradiation of the keratinocytes treated with the sequoia plant cells before, respectively by 18%, 24% and 27% and, after 3 hours of irradiation of 26%, 29% and 33%, compared to the non- treated control.
In the study of SOD activity, at
concentrations of 0.5%, 1% and 2.5%, after irradiation of the keratinocytes treated with the sequoia plant cells before, an increase of the SOD activity was shown respectively by 19%, 23% and 25%, after 30 min and, of 15%, 19% and 23%, after 3 hours, compared to the non-treated control. In the study of catalase activity, at concentrations of 0.5%, 1% and 2.5%, after irradiation of the keratinocytes treated with the sequoia plant cells before, an increase of the catalase activity was shown respectively by 22%, 24% and 31%, after 30 min, and of 18%, 21% and 24%, after 3 hours compared to the non-treated control.
Decreasing irritation Inflammation is the tissue’s answer to aggressions: all defence mechanisms through which they recognise, destroy and eliminate any foreign substances. Different types of cells take part in those mechanisms
but in the epidermis, it is the keratinocytes we will study.
At the beginning of inflammation, its
diffusion starts from the initial location and involves chemical factors that are locally synthetised or at the state of inactive precursors. Naolys decided to study three inflammation mediators synthetised at the level of the keratinocytes, 2 famous cytokines (IL1-apha and IL6) and a prostaglandine (PGE2). The results are shown in Figure 7.
Conclusion
MReload Sequoia (INCI: Sequoia Sempervirens Leaf Cell Extract) decreases deep and superficial wrinkles on the face, including mature skins, especially crow’s feet. Its moisturising effect keeps water in the whole epidermis and strengthens the cutaneous barrier.
As an antioxidant, MReload Sequoia
reduces the formation of free radicals, strengthens internal antioxidant protection, especially the action of antioxidant enzymes. At the same time, it offers a calming
effect, reducing temporary inflammatory processes and contributes to densify the dermis. This helps to improve or restore the dermis functions and skin resistance. MReload Sequoia can be used in
products such as creams, oils, masks, serums, essences, etc. Any cosmetic product for men intended to strengthen skin and limit skin ageing, and is for all skin types.
PC
+25% +23%
+19%
+23%
+19% +15%
T3h
Negative control
UVB MRS (0.5%) +UVB
MRS (1%) +UVB
MRS (2.5%) +UVB
Figure 7: Study of the inflammation mediators. November 2019 PERSONAL CARE ASIA PACIFIC
Negative control
UVB MRS (0.5%) +UVB
MRS (1%) +UVB
MRS (2.5%) +UVB
Negative control
UVB MRS (0.5%) +UVB
MRS (1%) +UVB
MRS (2.5%) +UVB
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92 |
Page 93 |
Page 94 |
Page 95 |
Page 96 |
Page 97 |
Page 98 |
Page 99 |
Page 100 |
Page 101 |
Page 102 |
Page 103 |
Page 104 |
Page 105 |
Page 106 |
Page 107 |
Page 108 |
Page 109 |
Page 110 |
Page 111 |
Page 112 |
Page 113 |
Page 114 |
Page 115 |
Page 116 |
Page 117 |
Page 118 |
Page 119 |
Page 120
