ANTI-AGEING 19


Figure 3: Olea Vitae PLF recharges the skin’s internal batteries.


ending up in the formation and rebalance of these mitochondrial synapses; it protects and optimises the skin cells’ energy, and increases the production of structural proteins, to obtain an anti-wrinkle, firming and repairing effect. Several clinical and efficacy tests were performed to support these claims:


In vitro efficacy In vitro tests were done to demonstrate the effect that the active has over the increase of the number of mitochondria, and their interactions, as well as the effect that this process has on the skin. These tests demonstrate that the active is able to enhance the interrelation between mitochondria, increases their number and functionality, maintains the health of the mitochondrial genetic pool and facilitates their protection and recovery in unfavourable conditions. Consequently, revitalised cells produce more energy and relevant biomolecules such as collagen.


ACP: Acyl Carrier protein. Mitochondrial signaling fatty acid pathway key enzyme


600 500 400 300 200 100 0


Control (untreated) 1.8-fold 1.3-fold


These tests demonstrate the ingredient’s ability to interact at a cellular level with the signalling lipids generation pathway in mitochondria, and the increase of key molecules for mitochondrial fusion.


Gene expression – quantitative RT-PCR To study the effect that the active ingredient has on the gene expression of genes related to energy production and mitochondrial fusion, the expression of the various genes was analysed through quantitative Real Time PCR (RT-qPCR) in Human Dermal Fibroblasts (HDF). The gene expression of non-treated HDF was compared to the expression of HDF treated with estradiol 2 µM during 24h and with the expression of HDF treated with 0.1% Olea VitaePLF


during 24h.


l ACP: Acyl Carrier protein: Key enzyme in the alternative pathway of synthesis of mitochondrial signalling lipids Recently, it has been discovered that in mitochondria there is an alternative


MFN2 Mitofusin 2. Mitochondrial fusion key protein


600 500 400 300 200 100 0


Oestradiol 2 uM Olea Vitae PLF 0.1% Control (untreated) 5-fold 4-fold


pathway of synthesis of specific fatty acids. There is a group of enzymes present in mitochondria, mtFASII (mitochondrial Fatty Acids Synthase 2), that are believed to be responsible of mitochondrial intercommunication. This enzymatic


complex has a role in mitochondrial fusion.7, 8, 9, 10


The active stimulates the expression of


ACP, one of the key enzymes in the mtFASII pathway, that increases the synthesis of these signalling lipids, directly related to mitochondrial fusion. This is a new mechanism of action that has not been described before. l MFN2: Mitofusin 2 – Key protein in mitochondrial fusion


Mitofusin 2 is a very important protein in mitochondrial fusion since it is responsible for recognising the lipid RAFTs in the mitochondrial membranes, and mechanistically, initiate the fusion process. Results show that the active interferes in a very specific way with the mitochondrial fusion process, increasing the gene


Oestradiol Figure 4: Gene expression of ACP, MFN2 genes linked to the production of energy and mitochondrial fusion. November 2019 PERSONAL CARE ASIA PACIFIC


2 uM


Olea Vitae PLF


0.1%


ACP


MFN2


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52  |  Page 53  |  Page 54  |  Page 55  |  Page 56  |  Page 57  |  Page 58  |  Page 59  |  Page 60  |  Page 61  |  Page 62  |  Page 63  |  Page 64  |  Page 65  |  Page 66  |  Page 67  |  Page 68  |  Page 69  |  Page 70  |  Page 71  |  Page 72  |  Page 73  |  Page 74  |  Page 75  |  Page 76  |  Page 77  |  Page 78  |  Page 79  |  Page 80  |  Page 81  |  Page 82  |  Page 83  |  Page 84  |  Page 85  |  Page 86  |  Page 87  |  Page 88  |  Page 89  |  Page 90  |  Page 91  |  Page 92  |  Page 93  |  Page 94  |  Page 95  |  Page 96  |  Page 97  |  Page 98  |  Page 99  |  Page 100  |  Page 101  |  Page 102  |  Page 103  |  Page 104  |  Page 105  |  Page 106  |  Page 107  |  Page 108  |  Page 109  |  Page 110  |  Page 111  |  Page 112  |  Page 113  |  Page 114  |  Page 115  |  Page 116  |  Page 117  |  Page 118  |  Page 119  |  Page 120