58 ANTI-AGEING *p<0.05 versus control 150 * +27.5% 100 n Control n 0.01% lodobacter ssp. extract 250 200 150 100 50 50 * 0 Control healthy 0 AD cells


Figure 2: ATP levels in AD-fibroblasts upon treatment with Iodobacter ssp. extract relative to untreated cells.


survival as they are able to adapt to conditions generally considered as hostile to life. There are prokaryotes, e.g. archaea and bacteria, but also eukaryotes and even metazoans which survive in extreme conditions such as very high or low temperatures, pH values, salt


Figure 3: Quantification of MAM contact points in healthy cells or AD- fibroblasts treated with the Iodobacter active or left untreated.


concentrations or pressures, and even ionizing or UV radiation. In order to adapt to such conditions, extremophiles developed various strategies which help them to cope with these stresses. For psychrotolerant bacteria, which are able to proliferate at temperature as low as 5°C,


survival is amongst others ensured by the following strategies: 1) incorporation of polyunsaturated fatty acids into the cell membrane in order to maintain membrane fluidity, 2) reduction of the freezing point of the intracellular fluid by addition of solutes such as trehalose, 3) expression of enzymes that are active even at low temperatures, and 4) production of a large number of secondary metabolites with various functions.10,11


These strategies could on the


Red: MAM contact points


Blue: cell nuclei


one hand be harnessed by the modern industry due to their biotechnological potential, and on the other hand psychrotolerant bacteria themselves represent interesting novel sources for secondary metabolites for cosmetic or pharmaceutical application.


A Swiss glacier bacterium for refreshing tired skin


AD cells


The continuous shrinking of glaciers in the past decades made more and more microbes accessible that have been hidden below permanent ice for centuries. In order to discover and harvest novel extremophile microorganisms for use in cosmetics, an expedition to a glacier in Valais, Switzerland, was undertaken. A sample of the soil exposed underneath the glacier was analysed for its microbial content, leading to the identification of Iodobacter ssp. After many years below the glacier ice layer, this cold-tolerant, rod-shaped bacterium has been reawakened and harnessed for the development of a novel active ingredient for skin care. Large-scale cultivation under optimised conditions and extraction of the Iodobacter ssp. strain followed by spray-drying the extract on a maltodextrin carrier yields the active ingredient IceAwakeTM


[INCI: Succinic Acid


Figure 4: Immunofluorescence staining of MAM contact points in healthy cells or AD-fibroblasts treated with the Iodobacter active or left untreated.


PERSONAL CARE ASIA PACIFIC


(and) Maltodextrin (and) Aqua/Water]. The efficacy of this novel active to reduce ER stress as a cause of prematurely aged and tired skin was investigated in vitro and in vivo.


November 2019 Control


0.01% lodobacter ssp. extract


*p<0.05 versus control AD cells


ATP production compared control cells (=100) in %


0.01% lodobacter ssp. extract


Control


Control healthy


Change in protein interactions comparedto healthy cells (=100) in %


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